Combretastatin A-4 phosphate prodrug mono- and di-organic amine salts, mono- and di-amino acid salts, and mono- and di-amino acid ester salts

ABSTRACT

Provided herein are novel and useful combretastatin A-4 prodrug salts that increase the solubility of combretastatin A-4, readily regenerate combretastatin A-4 in vivo under normal physiological conditions, and which produce physiologically tolerable products as a result of the regeneration of combretastatin A-4.

RELATED APPLICATIONS

This application is a continuation application of U.S. application Ser.No. 11/056,452, filed on Feb. 11, 2005, which is a divisional of U.S.application Ser. No. 10/660,439, filed on Sep. 10, 2003 now U.S. Pat.No. 6,855,702, which is a continuation of U.S. application Ser. No.09/950,500, filed Sep. 11, 2001 now U.S. Pat. No. 6,670,344, whichclaims priority from U.S. Application Ser. No. 60/232,568, filed Sep.14, 2000, and from U.S. Application Ser. No. 60/251,921, filed Dec. 7,2000, all of which are incorporated herein by reference in theirentirety.

FIELD OF THE INVENTION

The present invention relates to novel and useful combretastatin A-4(CA4) phosphate prodrug mono- and di-organic amine salts, mono- anddi-amino acid salts, and mono- and di-amino acid ester salts, whichsalts have greater solubility than native combretastatin A-4, and 15rapidly regenerate combretastatin A-4 under physiological conditions.

BACKGROUND OF THE INVENTION

Cancer is considered to be a serious and pervasive disease. The NationalCancer Institute has 20 estimated that in the United States alone, 1 in3 people will be struck with cancer during their lifetime. Moreoverapproximately 50% to 60% of people contracting cancer will eventuallysuccumb to the disease. Hence, since the establishment the NationalCancer Institute in the early 1970's, the amount of resources committedto cancer research has dramatically improved.

Although cancer is commonly considered to be a single disease, itactually comprises a family of diseases wherein normal celldifferentiation is modified so that it becomes abnormal anduncontrolled. As a result, these malignant cells rapidly proliferate.Eventually, the cells spread or metastasize from their origin andcolonize other organs, eventually killing their host. Due to the widevariety of cancers presently observed, numerous strategies have beendeveloped to destroy cancer within the body. One such method utilizescytotoxic chemotherapeutics. These compounds are administered to cancersufferers with the objective of destroying malignant cells while leavingnormal, healthy cells undisturbed. Particular examples of such compoundsinclude 5-fluorouracil, cisplatin, and methotrexate.

Combretastatin A-4 was initially isolated from stem wood of the Africantree combretum caffrum (Combretaceae), and found to be a potentinhibitor of microtubulin assembly. Moreover, combretastatin A-4 wasfound to be significantly active against the US National CancerInstitute's (NCI) murine L1210 and P338 lymphocytic leukemia cell lines.In addition, combretastatin A-4 was found to compete with combretastatinA-1, another compound isolated from Combretum caffrum, as an inhibitorof colchicine binding to tubulin. It has also been determined to retardstrongly the VoLo, DLD-1 and HCT-15 human-colon cancer (ED₅₀<0.01(μg/ml) cell lines, and to be one of the stronger anti-mitotic agentsfound among the Combretum caffrum constituents (U.S. Pat. No.4,996,237).

Consequently, research has been conducted to determine the efficacy ofcombretastatin A-4 as a chemotherapeutic in treating a variety of humancancers. Unfortunately, combretastatin A-4 is essentially insoluble inwater. This characteristic has significantly interfered with thedevelopment of pharmaceutical compositions comprising combretastatinA-4. In order to increase its solubility as well as its efficacy,efforts have been made to create prodrug derivatives of combretastatinA-4 that will regenerate combretastatin A-4 in physiological conditions.For example, Koji Ohsumi et al. describe the synthesis of amino acid HClprodrugs of a combretastatin analog, wherein an amino acid salt isattached to the amino group of a combretastatin derivative containing abasic amino group [the derivatives are described in Ohsumi et al,Anti-Cancer Drug Design, 14, 539-548 (1999)]. Although such prodrugs mayhave an increased solubility compared to native combretastatin A-4, theypossess an inherent limitation in that the regeneration ofcombretastatin A-4 is dependent upon endogenous aminopeptidase in theblood of a subject to whom the prodrug is administered.

The free acid of combretastatin A-4 phosphate (“CA4P free acid”) whichhas the following structure:

exists as an oily mass. The CA4P free acid is intrinsically VerySlightly Soluble (by USP definition) at 25° C. in water, with theaqueous solubility increasing with an increase in pH. It has two acidicgroups with pK_(a) values of 1.2 and 6.2, which are amenable to saltformation. As there are practical issues with handling CA4P free aciddue to its physical state, identification of a crystalline, stable saltform of this compound is desirable.

Attempts at derivatizing combretastatin A-4 have involved forming saltderivatives of combretastatin A-4 phosphate (salt derivatives of“CA4P”). Particular examples of such salts are set forth in U.S. Pat.No. 5,561,122. Although these prodrug salts possess greater solubilitythan native combretastatin A-4, they can also possess inherentdrawbacks, such as hygroscopicity.

Hygroscopicity is one of several important criteria in the selection ofa salt. See K. Morris et al., “An Integrated Approach to the Selectionof Optimal Salt Form for a New Drug Candidate”, Int. J. Pharm., 105,209-217 (1994). The extent of hygroscopicity for any drug substance hassignificant impact on its handling and stability over the lifetime ofthe drug product.

Accordingly, what is needed are novel and useful combretastatin A-4prodrug salts with favorable physiochemical properties, and whichincrease the solubility, and preferably efficacy, of combretastatin A-4in treating a wide variety of neoplastic disorders.

What is also needed are novel and useful combretastatin A-4 prodrugsalts which readily regenerates native combretastatin A-4 in vivo and donot produce unwanted or potentially deleterious side products whenundergoing regeneration.

The citation of any reference herein should not be construed as anadmission that such reference is available as “Prior Art” to the instantapplication.

SUMMARY OF THE INVENTION

There is provided, in accordance with the present invention, novel anduseful combretastatin A-4 phosphate prodrug mono- and di-organic aminesalts, mono- and di-amino acid salts, and mono- and di-amino acid estersalts, which salts have greater solubility than native combretastatinA-4, and readily regenerate combretastatin A-4 in vivo. The presentinvention also provides compounds which are significantly lesshygroscopic than heretofore known combretastatin A-4 prodrug salts (forexample, do not undergo significant changes in physical form underambient conditions of temperature and humidity), have improved handlingand stability, and which can form solutions at a pH which minimizes oreliminates pain at the injection site. The compounds of the inventionthus provide considerable advantages for pharmaceutical use.

Broadly, the present invention extends to a compound having a generalstructure of the formula I:

wherein one of —OR¹ or —OR² is —O—QH⁺, and the other is hydroxyl or—O—QH⁺; and Q is(A) an organic amine containing at least one nitrogen atom which,together with a proton, forms a quaternary ammonium cation QH⁺;(B) an amino acid containing at least two nitrogen atoms where one ofthe nitrogen atoms, together with a proton, forms a quaternary ammoniumcation QH⁺; or(C) an amino acid containing one or more nitrogen atoms where one of thenitrogen atoms, together with a proton, forms a quaternary ammoniumcation QH⁺ and where, further, all carboxylic acid groups of the aminoacid are in the form of esters.

Throughout this specification, when both —OR¹ and —OR² are —O—QH⁺, Q ispreferably identical in both the —OR¹ and —OR² groups.

When Q has definition (A), preferred organic amine having applicationsherein is tromethamine (i.e., tris(hydroxymethyl)aminomethane,abbreviated herein as “TRIS”).

Tromethamine salts of the formula I are illustrated by the followingformulae Ia and Ib which represent the mono-tromethamine anddi-tromethamine salts of formula I, respectively:

The mono-tromethamine salt of formula Ia is preferred.

When Q has definition (B), any amino acid having at least two nitrogenatoms has applications herein. Any of the nitrogens of the amino acidcan form the quaternary ammonium cation of formula I, for example, anynitrogen on the amino acid side chain or the nitrogen of the α-aminogroup. Amino acids having applications herein include, but certainly arenot limited to ornithine, histidine, lysine, arginine, tryptophan, etc.

When Q has definition (B), a preferred amino acid having applicationsherein is histidine. For example, either of the nitrogens of theimidazole group of the histidine side chain, or alternatively, thenitrogen of the α-amino group of histidine can form the quaternaryammonium cation of the formula I. As is readily apparent, due to thearomatic nature of the imidazole group, either of the nitrogens of theimidazole group of the histidine sidechain can form the structure of theformula (I). Preferred mono-histidine structures of the formula I areillustrated by the following formulae Ic or Id:

When Q has definition (C), any amino acid has applications herein, suchas, but not limited to glycine. Preferred esters are alkyl esters, suchas methyl or ethyl esters.

Furthermore, the present invention extends to a pharmaceuticalcomposition comprising:

(a) a compound having a general structure of the formula I:

wherein:

-   -   one of —OR¹ or —OR² is —O—QH⁺, and the other is hydroxyl or        —O—QH⁺; and Q is        (A) an organic amine containing at least one nitrogen atom        which, together with a proton, forms a quaternary ammonium        cation QH⁺;        (B) an amino acid containing at least two nitrogen atoms where        one of the nitrogen atoms, together with a proton, forms a        quaternary ammonium cation QH⁺; or        (C) an amino acid containing one or more nitrogen atoms where        one of the nitrogen atoms, together with a proton, forms a        quaternary ammonium cation QH⁺ and where, further, all        carboxylic acid groups of the amino acid are in the form of        esters; and        (b) a pharmaceutically acceptable carrier thereof.

Particular examples of compounds of the present invention havingapplications in such a pharmaceutical composition are described above.Moreover, any suitable pharmaceutically acceptable carrier hasapplications in a pharmaceutical composition of the present invention.Particular examples are described infra. A particular embodiment of apharmaceutical composition of the present invention comprises a compoundof the present invention in which tromethamine is the organic amine, andwhich is preferably a tromethamine salt having the structure of formulaIa or Ib, most preferably Ia:

Another particular embodiment of a pharmaceutical composition of thepresent invention comprises a compound of the present invention in whichhistidine is the amino acid, and which is preferably a mono-histidinesalt having the structure of formula Ic or Id:

Naturally, such a pharmaceutical composition would further comprise apharmaceutically acceptable carrier thereof.

In addition, the present invention further extends to compositionscomprising a salt of the present invention. In particular, a compositionof the present invention can be formed by mixing compounds comprising:

(a) a CA4P free acid having the structure:

(b) a compound Q, wherein Q is(A) an organic amine containing at least one nitrogen atom which iscapable of forming, together with a proton, a quaternary ammonium cationQH⁺;(B) an amino acid containing at least two nitrogen atoms where one ofthe nitrogen atoms, together with a proton, forms a quaternary ammoniumcation QH⁺; or(C) an amino acid containing one or more nitrogen atoms where one of thenitrogen atoms, together with a proton, forms a quaternary ammoniumcation QH⁺ and where, further, all carboxylic acid groups of the aminoacid are in the form of esters.

Optionally, a composition of the present invention can further comprisea pharmaceutically acceptable carrier.

Moreover, when Q has definition (A), any organic amine as defined hereinhas applications in a composition of the present invention. Particularexamples include, but are not limited to, tromethamine, diethanolamine,glucamine, N-methylglucamine, ethylenediamine, and 2-4-imidazolyl) ethylamine. When Q has definition (B), any amino acid having at least twonitrogen atoms has applications in a composition of the presentinvention. Particular examples include ornithine, histidine, lysine,arginine, tryptophan, etc. When Q has definition (C), any amino acidester as defined herein has applications in a composition of the presentinvention. Particular examples include glycine.

In another embodiment, the present invention extends to a method ofmodulating tumor growth or metastasis in an animal comprising theadministration of an amount effective therefor of a compound having ageneral structure of:

wherein:

-   -   one of —OR¹ or —OR² is —O—QH⁺, and the other is hydroxyl or        —O—QH⁺; and Q is        (A) an organic amine containing at least one nitrogen atom        which, together with a proton, forms a quaternary ammonium        cation QH⁺;        (B) an amino acid containing at least two nitrogen as where one        of the nitrogen atoms, together with a proton, forms a        quaternary ammonium cation QH⁺; or        (C) an amino acid containing one or more nitrogen atoms where        one of the nitrogen atoms, together with a proton, forms a q        ammonium cation QH⁺ and where, further, all carboxylic acid        groups of the amino acid are in the form of esters.

In a particular embodiment, the present invention extends to a method ofmodulating tumor growth or metastasis in an animal comprising theadministration of an amount effective therefor of a compound in whichtromethamine is the organic amine, and which is preferably atromethamine salt having the structure of formula Ia or Ib, mostpreferably, Ia:

In another particular embodiment, the present invention extends to amethod of modulating tumor growth or metastasis in an animal comprisingthe administration of an amount effective therefore of a compound inwhich histidine is the amino acid, and which is preferably amono-histidine salt having the structure of formula Ic or Id:

Accordingly, the present invention provides novel and usefulcombretastatin A4 phosphate prodrug mono- and di-organic amine salts,mono- and di-amino acid salts and mono- and di-amino acid ester salts,which salts are more soluble in aqueous solutions than nativecombretastatin A-4. Thus, the efficacy of this drug can be increased.

The present invention also provides combretastatin A4 phosphate prodrugmono- and di-organic amine salts, mono and di-amino acid salts and mono-and di-amino acid ester salts, which salts readily regeneratecombretastatin A-4 in vivo, and which, upon dissociation, liberate anorganic amine, amino acid or amino acid ester as a physiologicallytolerable byproduct.

In its most preferred embodiment, the present invention provides thenovel crystalline 1:1 tromethamine (TRIS) salt of the antivascularantitumor agent combretastatin A-4 phosphate prodrug having thestructure of formula Ia. This compound is a phosphate ester prodrugsalt, wherein the phosphate moiety undergoes dephosphorylation underphysiological conditions to yield the active drug moiety, combretastatinA-4 (as mentioned below, the olefin group bridging the phenyl moietiesof the core of combretastatin A-4 is in the cis configuration; theolefin group of the preferred mono TRIS salt of combretastatin A-4phosphate is similarly in the cis configuration). The 1:1 (mono) TRISsalt of CA4P exhibits good solid-state properties and is unexpectedlypractically nonhygroscopic. This and other favorable properties make theTRIS salt of CA4P a preferred compound for pharmaceutical dosageformulation.

BRIEF DESCRIPTION OF THE DRAWINGS

FIG. 1 shows the moisture sorption/desorption profile of the mono-TRISsalt of CA4P (prepared in Example 1) at 25° C. The data was obtainedusing a VTI Model MB-300W Moisture balance with relative humidity levelsfrom 100% to 90% in increments of 10%. The maximum equilibration time ateach humidity was set at 4 hours.

FIG. 2 shows the powder X-ray diffraction patterns of samples of themono-TRIS salt of CA4P (prepared in Example 1), which were slurried indifferent solvents (water, isopropanol, ethanol, acetonitrile andacetone) initially at 70 to 75° C. for 5 to 10 minutes, and then at roomtemperature overnight. The X-ray diffractograms were recorded using aRigaku Model Miniflex X-ray diffractometer with a Cu-Kα source at a scanrate of 1° per minute from 2° to 40° 2θ.

FIG. 3 shows the differential scanning calorimetry (DSC) thermogram(Model DSC 2910, TA instruments) of the mono-TRIS salt of CA4P (preparedin Example 1) obtained under flow of nitrogen with a heating rate of 10degrees per minute.

FIG. 4 shows the pH-solubility profile of the mono-TRIS salt of CA4P(prepared in Example 1) at 25° C. The pH was adjusted with sodiumhydroxide.

FIG. 5 shows the differential scanning calorimetry (DSC) thermogram ofthe mono-L-histidine salt of CA4P (prepared in Example 3) (2.0900 mgsample size).

FIG. 6 shows the differential scanning calorimetry (DSC) thermogram ofthe mono-L-histidine salt of CA4P (prepared in Example 3).

FIG. 7 shows the differential scanning calorimetry (DSC) thermogram ofthe mono-L-histidine salt of CA4P (prepared in Example 3).

FIG. 8 shows the powder X-ray diffraction patterns of themono-L-histidine salt of CA4P (prepared in Example 3).

FIG. 9 shows the powder X-ray diffraction patterns of themono-L-histidine salt of CA4P (prepared in Example 3).

FIG. 10 shows the differential scanning calorimetry (DSC) thermogram ofthe mono-L-histidine anhydrous salt of CA4P (prepared in Example 3).

FIG. 11 shows the powder X-ray diffraction patterns of themono-L-histidine anhydrous salt of CA4P (prepared in Example 3).

FIG. 12 shows the differential scanning calorimetry (DSC) thermogram ofthe CA4P monoglycine methyl ester salt (prepared in Example 4).

FIG. 13 shows the powder X-ray diffraction patterns of the CA4Pmonoglycine methyl ester salt (prepared in Example 4).

DETAILED DESCRIPTION OF THE INVENTION

The present invention is based upon the discovery that surprisingly andunexpectedly, mono- and di-organic amine, mono- and di-amino acid andmono- and di-amino acid ester combretastatin A-4 phosphate prodrug saltscan be formed that have increased solubility in vivo relative to thesolubility of native combretastatin A-4, readily regeneratecombretastatin A-4 under physiological conditions, and duringregeneration, produce physiologically tolerable organic amines, orphysiologically tolerable amino acids or amino acid esters that arereadily metabolized in vivo.

Broadly, the present invention extends to a compound having a generalstructure of:

wherein:

-   -   one of —OR¹ or —OR² is —O—QH⁺, and the other is hydroxyl or        —O—QH⁺; and Q is        (A) an organic amine containing at least one nitrogen atom        which, together with a proton, forms a quaternary ammonium        cation QH⁺;        (B) an amino acid containing at least two nitrogen atoms where        one of the nitrogen atoms, together with a proton, forms a        quaternary ammonium cation QH⁺; or        (C) an amino acid containing one or more nitrogen atoms where        one of the nitrogen atoms, together with a proton, forms a        quaternary ammonium cation QH⁺ and where, further, all        carboxylic acid groups of the amino acid are in the form of        esters.

All isomers of the present compounds (for example, those which may existdue to asymmetric carbons, such as on various substituents), includingenantiomeric forms and diastereomeric forms, are contemplated within thescope of this invention. Individual stereoisomers of the compounds ofthe invention may, for example, be substantially fee of other isomers(e.g., as a pure or substantially pure optical isomer having a specifiedactivity), or may be admixed, for example, as racemates or with allother, or other selected, stereoisomers. The chiral centers of theinvention may have the S or R configuration as defined by the IUPAC 1974Recommendations. The racemic forms can be resolved by physical methods,such as, for example, fractional crystallization, separation orcrystallization of diastereomeric derivatives or separation by chiralcolumn chromatography. The individual optical isomers can be obtainedfrom racemates by any suitable method. The olefin group bridging thephenyl moieties of the combretastatin A-4 core is in the cisconfiguration, which is the preferred configuration for compounds of thepresent invention. Use of the terms “combretastatin A-4” or “CA4” as thename of, or part of the name of, a compound herein denotes a compound inthis cis configuration. Solvates, such as hydrates, of the compound offormula I are contemplated herein.

Throughout the specification, groups and substituents may be chosen toprovide stable moieties and compounds.

Embodiments indicated herein as exemplary or preferred are intended tobe illustrative and not limiting.

In another embodiment, the present invention extends to a pharmaceuticalcomposition comprising a compound of the present invention, andpharmaceutically acceptable carrier thereof. Naturally, the compounds ofthe present invention can be employed is any form, such as a solid orsolution (particularly aqueous solution) form as is described furtherbelow. The compound, for example, can be obtained and employed in alyophilized form alone or with suitable additives.

In yet another embodiment, the present invention extends to a method ofmodulating tumor growth or metastasis in an animal comprising theadministration of an effective amount of a compound having a generalstructure of the formula I:

wherein:

-   -   one of —OR¹ or —OR² is —O—QH⁺, and the other is hydroxyl or        —O—QH⁺; and Q is        (A) an organic amine containing at least one nitrogen atom        which, together with a proton, forms a quaternary ammonium        cation QH⁺;        (B) an amino acid containing at least two nitrogen atoms where        one of the nitrogen atoms, together with a proton, forms a        quaternary ammonium cation QH⁺; or        (C) an amino acid containing one or more nitrogen atoms where        one of the nitrogen atoms, together with a proton, forms a        quaternary ammonium cation QH⁺ and where, further, all        carboxylic acid groups of the amino acid are in the form of        esters.

Naturally, a compound of the present invention can be administered aloneor in a pharmaceutical composition.

The terms and phrases used herein are defined below, and have theindicated definitions unless otherwise indicated.

As used herein the terms “modulate,” “modulating” or “modulation” referto changing the rate at which a particular process occurs, inhibiting aparticular process, reversing a particular process, and/or preventingthe initiation of a particular process. Thus, for example, where theparticular process comprises tumor growth or metastasis, “modulation” ofthe process includes decreasing the rate at which tumor growth and/ormetastasis occurs, inhibiting tumor growth and/or metastasis, reversingtumor growth and/or metastasis (including tumor shrinkage and/oreradication) and/or preventing tumor growth and/or metastasis,particularly in a subject that is prone to such a process.

As used herein, the phrase “effective amount” or “amount effectivetherefor” of a compound administered to an animal is that amountsufficient to modulate tumor growth or metastasis in an animal. One ofordinary skill in the art can readily determine an effective amount of acompound of the present invention to be administered to an animal, forexample, using routine techniques. Exemplary dosage amounts for an adulthuman are from about 0.05 to about 1000 mg/kg of body weight of activecompound per day, which can be administered in a single dose (forexample, as a bolus or as an infusion over time at or below the maximumtolerated dose) or in the form of individual divided doses (for example,as consecutive dosages below the maximum tolerated dose), such as from 1to 4 times per day. It will be understood that the specific dose leveland frequency of dosage for any particular subject can be varied andwill depend upon a variety of factors including the activity of thespecific compound employed, the metabolic stability and length of actionof that compound, the species, age, body weight, general health, sex anddiet of the subject, the mode and time of administration, rate ofexcretion, drug combination, and severity of the particular condition.

As used herein the term “animal” preferably includes subjects such asdomestic animals, and most preferably, humans.

As used herein the term “prodrug” refers to a precursor compound thatwill undergo metabolic activation in vivo to produce the active drug.Thus, for example, a compound of the invention administered to a subjectwill undergo metabolic activation and regenerate combretastatin A-4 dueto the dissociation of a compound of the present invention, for example,by the action of endogenous non-specific phosphatases in plasma.

As used herein, the term “organic amine” refers to an organic (i.e.,carbon-containing) compound containing at least one primary (i.e.,—NH₂), secondary (i.e., —NH—) or tertiary (i.e.,

amine group capable of forming a phosphate salt in the compound of theformula I of the present invention. Where there is more than oneprimary, secondary and/or tertiary amine group within said organicamine, any such group capable of doing so can form the quaternaryammonium group QH⁺ of formula I. The present definition of “organicamine” does not encompass amino acid compounds (see the provisionalapplication entitled “Combretastatin A-4 Phosphate Mono- and Di-AminoAcid Salt Prodrugs”, filed by Venit as U.S. Application Ser. No.60/232,568 on Sep. 14, 2000, incorporated herein by reference in itsentirety) nor certain compounds as described in WO 99/35150(glucosamine, piperazine, piperidine, 6′-methoxy-cinchonan-9-ol,cinchonan-9-ol, pyrazole, pyridine, tetracycline, imidazole, adenosine,verapamil, morpholine), incorporated herein by reference in itsentirety. The organic amine employed is preferably a physiologicallytolerable compound selected from the following groups:

-   -   (a) organic amines having a pK_(α) greater than or equal to 7,        more preferably a pK_(α) greater than or equal to 8;    -   (b) organic amines wherein the nitrogen forming the quaternary        ammonium cation QH⁺ in the formula I is bonded to an optionally        substituted aliphatic group or to an optionally substituted        heterocyclic non-aromatic group (or two or three such optionally        substituted aliphatic and/or heterocyclic non-aromatic groups in        the case of secondary or tertiary amines, respectively). The        “aliphatic group” is a straight or branched-chain, saturated or        unsaturated hydrocarbon (e.g., alkane, alkene or alkyne) having        from 1 to 20, preferably 1 to 12, more preferably 1 to 6 carbon        atoms in the chain. The “heterocyclic non-aromatic group” is a        saturated or partially unsaturated ring containing the nitrogen        forming the quaternary ammonium cation QH⁺ in the formula I as        well as, optionally, other heteroatoms in the ring such as O, S        or additional N atoms. “Optional substituents” are preferably        one or more substituents providing an organic amine which, when        employed in the formula I of the present invention, results in        phosphate salts of the formula I which are crystalline and        practically nonhygroscopic or nonhygroscopic. Preferred        “optional substituents” include hydroxyl, amino (i.e., —NH₂), or        alkoxy (i.e., —O-alkyl) groups, most preferably, one or more        hydroxyl groups; and/or    -   (c) organic amines wherein the nitrogen forming the quaternary        ammonium cation QH⁺ in the formula I is a primary amine bonded        to an optionally substituted aliphatic group or a secondary        amine bonded to two optionally substituted aliphatic groups,        wherein the preferred optional substituents are one or more        hydroxyl or amino groups, most preferably, hydroxyl groups.

Of course, any given organic amine selected as a preferred amine for usein the present invention may have the properties of two or more groups(a) to (c) described above (for example, have a pK_(a) greater than orequal to 7 and be an optionally substituted aliphatic amine as describedin (c)).

Any organic amine so defined is suitable for use in the compounds offormula I of the present invention, as well as the presentpharmaceutical compositions and methods. The term “organic amine”includes compounds in salt form with other acidic and/or basic moieties(where, for example, one amine group forms the phosphate salt of formulaI and another amine group forms a salt with an acidic moiety). Thus, theremainder of the organic amine comprised within a compound of thepresent invention can also contain salt moieties.

Exemplary organic amines include, but are certainly not limited to,tromethamine, diethanolamine, glucamine, N-methylglucamine,ethylenediamine, and 2-(4-imidazolyl) ethyl amine.

A combretastatin A-4 phosphate “mono-organic amine” salt of the formulaI contains one organic amine group Q as part of R¹ or R² as definedabove; a combretastatin A-4 phosphate “di-organic amine” salt of theformula I contains two organic amine groups Q, one part of R¹ and onepart of R² as defined above. “Mono-organic amine” salts of the formula Iare preferred. Corresponding definitions apply for “mono-amino acid”,“di-amino acid”, “mono-amino acid ester” and “di-amino acid ester”.

Any suitable amino acid has applications herein, including numerousnatural and non-natural amino acids having applications in a compound ofthe present invention. Particular examples include, but certainly arenot limited to ornithine, histidine, lysine, arginine, and tryptophan,to name only a few.

As used herein, the term “amino acid” refers to a compound containing abasic amino group (NH₂) and an acidic carboxylic acid group (COOH),including such compounds in zwitterionic form (where the amino andcarboxyl groups together form a zwitterion or internal salt), or in saltform with other acidic and/or basic moieties (where, for example, theamino acid contains a carboxylic acid group in addition to the α-COOHgroup, and the former is in salt form with an alkali metal). Thus, theremainder of an amino acid comprised within a compound of the presentinvention can also contain salt moieties. The term includes non-naturalas well as natural amino acids, such as α-amino acids (especiallyL-amino acids) many of which are the building blocks of proteins. Theterm “natural amino acids” refers to the 20 amino acids that are commonin all proteins, i.e., glycine, alanine, valine, leucine, isoleucine,proline, serine, threonine, cysteine, methionine, asparagine, glutamine,phenylalanine, tyrosine, tryptophan, lysine, arginine, histidine,aspartate, and glutamate. The phrase “non-natural amino acids” refers toamino acids that are generally not common in all proteins, such as4-hydroxyproline, 5-hydroxylysine, N-methyllysine, γ-carboxyglutamate,selenocystein, ornithine and citrulline. Amino acids having two or morenitrogen atoms are suitable for use in the compounds of formula I of thepresent invention when Q has definition (B), as well as the presentpharmaceutical compositions and methods.

As used herein, the phrase “side chain” with respect to an amino acid isthat moiety of an amino acid which is different for each amino acid,especially the group bonded to the carbon linking the —NH₂ and —COOHgroups of an amino acid.

As used herein, the term “practically nonhygroscopic”, in reference to acompound, preferably denotes less than 1% water weight gain per weightof compound (more preferably, less than 0.5% water weight gain perweight of compound) measured under the following conditions: atemperature of approximately 25° C., relative humidities of from 20% to95%, and at equilibrium conditions (i.e., measured at a time wherein therates of moisture sorption and desorption have equilibrated), relativeto measurement under 25° C. and 0% relative humidity conditions. As usedherein, the term “nonhygroscopic”, in reference to a compound,preferably denotes no measurable weight gain per weight of compound asmeasured above.

As used herein in reference to an amino acid, the term “ester” refers toa carboxylic acid group (i.e., a group —COOH) of the amino acid which isin the form —COO(G) where G is an organic moiety such as anunsubstituted or substituted alkyl, alkenyl, alkynyl, cycloalkyl,cycloalkenyl, aryl or heterocyclo group. Preferred as groups G are C₁₋₆alkyl groups such as methyl or ethyl. Most preferred as amino acidesters are C₁₋₆ alkyl esters of glycine, such as glycine methyl ester orglycine ethyl ester.

As used herein, the term “salt” refers to a compound of the presentinvention which is an ionic compound, for example, having an ionic bondbetween the quaternary nitrogen of the organic amine, amino acid oramino acid ester moiety QH⁺ and the phosphate moiety of combretastatinA4 phosphate.

As used herein, an “ionic bond” is a chemical bond that forms by theelectrostatic attraction between positive and negative ions or chemicalstructures. Such a bond can be readily dissociated (or ionized) in anaqueous solution. Dissolution of a compound of the present invention ina solvent, particularly an aqueous solvent, as well as thelyophilization of such a solution are embodiments encompassed by thepresent invention.

As used herein, the phrase “physiologically tolerable” in describing achemical specie present in vivo, such an organic amine, amino acid oramino acid ester, refers to the inability of the chemical specie toinduce side effects unacceptable under the conditions of treating theanimal. Preferably, “physiologically tolerable” chemical species produceno deleterious side effects.

As explained above, the present invention is directed towards a methodfor modulating the growth or metastasis of tumors, preferably solidtumors, using a compound of the present invention. As used herein, theterms “tumor” or “tumor growth” can be used interchangeably, and referto an abnormal growth of tissue resulting from uncontrolled progressivemultiplication of cells and serving no physiological function. A solidtumor can be malignant, e.g. tending to metastasize and being lifethreatening, or benign. Examples of solid tumors that can be treatedaccording to a method of the present invention include sarcomas andcarcinomas such as, but not limited to: fibrosarcoma, myxosarcoma,liposarcoma, chondrosarcoma, lymphoma, such as non-Hodgkin's lymphoma,osteogenic sarcoma, chordoma, esophageal tumors, angiosarcoma,osteosarcoma, endotheliosarcoma, lymphangiosarcoma,lymphangioendotheliosarcoma, synovioma, synovial sarcoma, mesothelioma,Ewing's tumor, leiomyosarcoma, rhabdomyosarcoma, colon carcinoma,colorectal cancer, gastric cancer, pancreatic cancer, breast cancer suchas metastatic breast cancer, ovarian cancer, prostate cancer, squamouscell carcinoma, basal cell carcinoma, adenocarcinoma, adenocarcinoma ofthe colon and rectum, sweat gland carcinoma, sebaceous gland carcinoma,papillary carcinoma, papillary adenocarcinomas, cystadenocarcinoma,medullary carcinoma, bronchogenic carcinoma, renal cell carcinoma,hepatoma, liver metastases, bile duct carcinoma, choriocarcinoma,seminoma, embryonal carcinoma, thyroid carcinoma such as anaplasticthyroid cancer, medullary thyroid tumor, Wilms' tumor, cervical cancer,testicular tumor, lung tumors such as non-small cell lung carcinoma,small cell lung carcinoma, bladder carcinoma, epithelial carcinoma,glioma, astrocytoma, medulloblastoma, craniopharyngioma, ependymoma,pinealoma, hemangioblastoma, acoustic neuroma, oligodendroglioma,meningioma, melanoma, neuroblastoma, and retinoblastoma.

Moreover, tumors comprising dysproliferative changes (such asmetaplasias and dysplasias) can be treated or prevented with a compoundor method of the present invention in epithelial tissues such as thosein the cervix, esophagus, and lung. Thus, the present invention providesfor treatment of conditions known or suspected of preceding progressionto neoplasia or cancer, in particular, where non-neoplastic cell growthconsisting of hyperplasia, metaplasia, or most particularly, dysplasiahas occurred (for review of such abnormal growth conditions, see Robbinsand Angell, 1976, Basic Pathology, 2d Ed., W.B. Saunders Co.,Philadelphia, pp. 68-79). Hyperplasia is a form of controlled cellproliferation involving an increase in cell number in a tissue or organ,without significant alteration in structure or function. As but oneexample, endometrial hyperplasia often precedes endometrial cancer.Metaplasia is a form of controlled cell growth in which one type ofadult or fully differentiated cell substitutes for another type of adultcell. Metaplasia can occur in epithelial or connective tissue cells.Atypical metaplasia involves a somewhat disorderly metaplasticepithelium. Dysplasia is frequently a forerunner of cancer, and is foundmainly in the epithelia; it is the most disorderly form ofnon-neoplastic cell growth, involving a loss in individual celluniformity and in the architectural orientation of cells. Dysplasticcells often have abnormally large, deeply stained nuclei, and exhibitpleomorphism. Dysplasia characteristically occurs where there existschronic irritation or inflammation, and is often found in the cervix,respiratory passages, oral cavity, and gall bladder. For a review ofsuch disorders, see Fishman et al., 1985, Medicine, 2d Ed., J. B.Lippincott co., Philadelphia.

Other examples of tumors that are benign and can be treated with acompound or method of the present invention include arteriovenous (AV)malformations, particularly in intracranial sites and myoleomas.

The compounds of the present invention are also useful in methods forthe treatment of non-malignant vascular proliferative disorders such asmacular degeneration, psoriasis and restenosis, and, in general, in thetreatment of inflammatory diseases characterized by vascularproliferation. Such diseases and disorders are described in WO 00/48606,incorporated herein by reference in its entirety.

Pharmaceutical Compositions

The present invention also extends to a pharmaceutical compositioncomprising a compound of the present invention as described above, and apharmaceutically acceptable carrier thereof. The phrase“pharmaceutically acceptable” refers to molecular entities andcompositions that are physiologically tolerable and preferably do nottypically produce an allergic or similar untoward reaction, such asgastric upset, dizziness and the like, when administered to a human.Preferably, as used herein, the term “pharmaceutically acceptable” meansapproved by a regulatory agency of the Federal or a State government orlisted in the U.S. Pharmacopeia or other generally recognizedpharmacopeia for use in animals, and more particularly in humans. Theterm “carrier” refers to a diluent, adjuvant, excipient, or vehicle withwhich the compound is administered. Such pharmaceutical carriers can besterile liquids, such as water and oils, including those of petroleum,animal, vegetable or synthetic origin, such as peanut oil, soybean oil,mineral oil sesame oil, alcohols, e.g., ethanol propanol, polyethyleneglycol, propylene glycol sorbitol, glycerine, etc., Cremophor and thelike, including mixtures thereof. Water or aqueous saline solutions andaqueous dextrose and glycerol solutions are preferably employed ascarriers, particularly for injectable solutions. Suitable pharmaceuticalcarriers are described in “Remington's Pharmaceutical Sciences” by E. W.Martin.

A pharmaceutical composition of the present invention can be foradministration for injection, or for oral, pulmonary, nasal,transdermal, ocular or other forms of administration. In generalcomprehended by the invention are pharmaceutical compositions comprisingeffective amounts of a compound of the present invention together with,for example, pharmaceutically acceptable diluents, preservatives,solubilizers, emulsifiers, adjuvants and/or other carriers. Suchcompositions include diluents of various buffer content (e.g., TRIS orother amines, carbonates, phosphates, amino acids, for example,glycinamide hydrochloride (especially in the physiological pH range),N-glycylglycine, sodium or potassium phosphate (dibasic, tribasic), etc.or TRIS-HCl or acetate), pH and ionic strength; additives such asdetergents and solubilizing agents (e.g., surfactants such as Pluronics,Tween 20, Tween 80 (Polysorbate 80), Cremophor, polyols such aspolyethylene glycol, propylene glycol, etc.), anti-oxidants (e.g.,ascorbic acid, sodium metabisulfite), preservatives (e.g., Thimersol,benzyl alcohol, parabens, etc.) and bulking substances (e.g., sugarssuch as sucrose, lactose, mannitol, polymers such aspolyvinylpyrrolidones or dextran, etc.); and/or incorporation of thematerial into particulate preparations of polymeric compounds such aspolylactic acid, polyglycolic acid, etc. or into liposomes. Hyaluronicacid may also be used Such compositions can be employed to influence thephysical state, stability, rate of in vivo release, and rate of in vivoclearance of a compound of the present invention. See, e.g., Remington'sPharmaceutical Sciences, 18th Ed (1990, Mack Publishing Co., Easton, Pa.18042) pages 1435-1712 which are herein incorporated by reference. Thecompositions can, for example, be prepared in liquid form, or can be indried powder, such as lyophilized form. Particular methods ofadministering such compositions are described infra.

pH adjustment can be employed as desired. It is preferred—for example,to enhance the solubility of compounds of the present invention—toadjust the pH of pharmaceutical compositions comprising these compoundsto a pH greater than 7, more preferably, to a pH greater than 8 (such asto a pH of about 8.5).

For CA4P prodrug salts such as those of formula I of the presentinvention, active parent (CA4) formation occurs at lower pH. The presentinventors have found that addition of a buffer/pH adjustment agentprevents pH drop during freezing thus providing a more stable lyophileformulation. It has further been surprisingly found that, for lyophileformulations of CA4P prodrug salts such as those of the formula I of thepresent invention, pH adjustment employing sodium hydroxide as the pHadjustment agent can result in the formation of the active parent (cis)CA4 (which is minimally soluble in water and can form undesirableparticulates in an aqueous solution), and that use of a pH adjustmentagent other than sodium hydroxide can mitigate CA4 formation. Use ofTRIS as a pH adjustment agent and buffer, for example, mitigates theformation of the active parent (cis) CA4 relative to the use of sodiumhydroxide for pH adjustment, and thus is particularly preferred for usein compositions of the present invention. For example, observations havebeen made for compounds of the formula I where Q is either TRIS orhistidine such that lyophiles prepared using NaOH as a pH adjustmentagent showed hydrolysis to parent cis-CA4 upon storage whereas pHadjustment using a suitable buffering agent other than NaOH, forexample, TRIS mitigated formation of the insoluble parent. Anotheraspect of the present invention therefore relates to pharmaceuticallyophile compositions (preferably prepared from pH-adjusted solutions),comprising a compound of the present invention and a pH adjustment agentother than sodium hydroxide, preferably, comprising an organic base suchas an amino acid or organic amine, especially TRIS, as the pH adjustmentagent. Thus, while use of sodium hydroxide is encompassed withinlyophile compositions of the present invention, such use is lesspreferred, for example, for pharmaceutical compositions to beadministered intravenously where solids formation is undesirable.

Pharmaceutical compositions, for example, solutions (especially aqueoussolutions, for example, at concentrations at 15 mg/mL, 30 mg/mL and 60mg/mL) can also be prepared comprising a compound of the presentinvention and a pH adjustment agent including sodium hydroxide,preferably, comprising an organic base such as an amino acid such asarginine, glycine, or organic amine, e.g. ethanolamine, especially TRIS,as the pH adjustment agent. The aqueous solution stability increases asthe pH of the formulation increases from pH 9.0 to pH 10.5. The solutionstability is also better at higher ionic strengths. For example, asolution formulation with NaOH as a pH adjusting agent at pH 10 canindicate comparable stability to the lyophilized formulation preparedwith TRIS as a buffering agent at pH 8.5.

Protection from light is preferably employed for the pharmaceuticalcompositions of the present invention.

Methods for Modulating Tumor Growth or Metastasis

Combretastatin A-4 is a very potent antimitotic agent derived from thestem wood of Combretum caffrum, and shows potent toxicity against a widevariety of human cancer cell lines. Consequently, administering acompound or pharmaceutical composition of the present invention to asubject can reduce tumor growth and/or metastasis in the subject oralternatively, if the subject has no detectable metastasis or tumorgrowth, will prevent metastasis and/or tumor growth. Naturally, acompound of the present invention can be administered alone or with apharmaceutically acceptable carrier.

Hence, the present invention is directed towards methods for modulatingtumor growth or metastasis comprising, inter alia, the administration ofan effective amount of a mono- or di-organic amine combretastatin A-4phosphate prodrug salt of the present invention that rapidly regeneratescombretastatin A-4 in vivo. As explained above, the phrase “effectiveamount” as used herein refers to the amount of compound of the presentinvention administered to a subject that is sufficient to modulate tumorgrowth or metastasis, such as to decrease tumor growth and metastasis inan animal or alternatively to prevent formation of tumor growth in ananimal that lacked any tumor formation prior to administration. One ofordinary skill in the art can readily determine the effective amount ofa compound of the present invention to administer, for example, usingroutine techniques.

Moreover, numerous means for administrating a compound of the presentinvention have applications in a method of the present invention. Inparticular, a compound or pharmaceutical composition of the presentinvention can be introduced parenterally, transmucosally, e.g., orally,nasally, or rectally, or transdermally. Preferably, administration isparenteral, e.g., via intravenous injection, and also including, but notlimited to, intra-arteriole, intramuscular, intradermal, subcutaneous,intraperitoneal, intraventricular, and intracranial administration. Thecompound or pharmaceutical composition can, for example, be introducedby injection into the tumor(s) being treated or into tissues surroundingthe tumor(s). “Mucosal penetration enhancer” refers to a reagent thatincreases the rate or facility of transmucosal penetration of a compoundof the present invention, such as but not limited to, a bile salt, fattyacid, surfactant or alcohol. In specific embodiments, the permeationenhancer can be sodium cholate, sodium dodecyl sulfate, sodiumdeoxycholate, taurodeoxycholate, sodium glycocholate, dimethylsulfoxideor ethanol. Suitable penetration enhancers also include glycyrrhetinicacid (U.S. Pat. No. 5,112,804 to Kowarski) and polysorbate-80, thelatter preferably in combination with a non-ionic surfactant such asnonoxynol-9, laureth-9, poloxamer-124, octoxynol-9, or lauramide-DEA(European Patent EP 0 242 643 B1 by Stoltz).

In another embodiment, according to a method of the present invention, acompound or pharmaceutical composition of the present invention can bedelivered in a vesicle, in particular a liposome [see Langer, Science249:1527-1533 (1990); Treat et al., in Liposomes in the Therapy ofInfectious Disease and Cancer, Lopez-Berestein and Fidler (eds.), Liss:New York, pp. 353-365 (1989); Lopez-Berestein, ibid., pp. 317-327; seegenerally ibid.].

In yet another embodiment, such a compound or pharmaceutical compositioncan be delivered in a controlled release system, such as using anintravenous infusion, an implantable osmotic pump, a transdermal patch,liposomes, or other modes of administration. In a particular embodiment,a pump may be used [see Langer, supra; Sefton, CRC Crit. Ref. BiomedEng. 14:201 (1987); Buchwald et al., Surgery 88:507 (1980); Saudek etal., N. Engl. J. Med. 321:574 (1989)]. In another embodiment, polymericmaterials can be used [see Medical Applications of Controlled Release,Langer and Wise (eds.), CRC Press: Boca Raton, Fla. (1974); ControlledDrug Bioavailability, Drug Product Design and Performance, Smolen andBall (eds.), Wiley: New York (1984); Ranger and Peppas, J. Macromol.Sci. Rev. Macromol. Chem. 23:61 (1983); see also Levy et al., Science228:190 (1985); During et al., Ann. Neurol. 25:351 (1989); Howard etal., J. Neurosurg. 71:105 (1989)]. In yet another embodiment, acontrolled release system can be placed in proximity of the targettissues of the subject, thus requiring only a fraction of the systemicdose [see, e.g., Goodson, in Medical Applications of Controlled Release,supra, vol. 2, pp. 115-138 (1984)]. Preferably, a controlled releasedevice is introduced into an animal in proximity of the site ofinappropriate immune activation or a tumor. Other controlled releasesystems are discussed in the review by Langer [Science 249:1527-1533(1990)].

Parenteral Administration

As explained above, a compound or pharmaceutical composition of thepresent invention can be administered parenterally to a subject, andthus avoid administration via a subject's gastrointestinal tract.Particular parenteral administration techniques having applicationsherein include, but certainly are not limited to intravenous (bolus orinfusion) injections, intraperitoneal injections, subcutaneousinjections, intramuscular injections, or catheterizations, to name onlya few. Exemplary compositions for parenteral administration includeinjectable solutions or suspensions which can contain, for example,suitable non-toxic, parenterally acceptable diluents or solvents, suchas mannitol, 1,3-butanediol, water, buffered aqueous systems, Ringer'ssolution, an isotonic sodium chloride solution, or other suitabledispersing or wetting and suspending agents, including synthetic mono-or diglycerides, and fatty acids, including oleic acid, alcohols, and/orCremophor. pH adjustment can be employed as desired.

Nasal Delivery

Nasal or transmucosal delivery of a compound or pharmaceuticalcomposition of the present invention is also contemplated. Nasaldelivery allows the passage of such a compound to the blood steamdirectly after administering an effective amount of the compound to thenose, without the necessity for deposition of the product in the lung.Formulations for nasal delivery include those with dextran orcyclodextrin, as well as other polymers such as polyvinylpyrrolidones,methyl cellulose or other celluloses.

For nasal administration, a useful device is a small hard bottle towhich a metered dose sprayer is attached. In one embodiment, the metereddose is delivered by drawing a compound or pharmaceutical composition ofthe present invention into a chamber of defined volume, which has anaperture dimensioned to aerosolize an aerosol formulation by forming aspray when a liquid in the chamber is compressed. The chamber iscompressed to administer the compound or pharmaceutical composition. Ina specific embodiment, the chamber is a piston arrangement. Such devicesare commercially available.

Alternatively, a plastic squeeze bottle having an aperture or openingdimensioned to aerosolize an aerosol formulation can be used.Aerolsolization occurs when the bottle is squeezed. The opening isusually found in the top of the bottle, and the top is generally taperedto partially fit in the nasal passages for efficient administration ofthe aerosol formulation. Preferably, the nasal inhaler will provide ametered amount of the aerosol formulation, for administration of ameasured dose of a compound or pharmaceutical composition of the presentinvention.

For transmucosal delivery, use of permeation enhancers is alsocontemplated.

Pulmonary Delivery

Also contemplated herein is pulmonary delivery of a compound orpharmaceutical composition of the present invention. A compound orpharmaceutical composition of the present invention can be delivered tothe lungs of a mammal while inhaling and traverses across the lungepithelial Lining to the blood stream. Other reports of this includeAdjei et al. [Pharmaceutical Research, 7:565-569 (1990); Adjei et al.,International Journal of Pharmaceutics, 63:135-144 (1990) (leuprolideacetate); Braquet et al., Journal of Cardiovascular Pharmacology,13(suppl. 5):143-146 (1989) (endothelin-1); Hubbard et al., Annals ofInternal Medicine, Vol. III, pp. 206-212 (1989) (α1-antitrypsin); Smithet al., J. Clin. Invest. 84: 1145-1146 (1989) (α-1-proteinase); Osweinet al., “Aerosolization of Proteins”, Proceedings of Symposium onRespiratory Drug Delivery II, Keystone, Colo., March, (1990)(recombinant human growth hormone); Debs et al., J. Immunol.140:3482-3488 (1988) (interferon-γ and tumor necrosis factor alpha),Platz et al., U.S. Pat. No. 5,284,656 (granulocyte colony stimulatingfactor)]. A method and composition for pulmonary delivery of drugs forsystemic effect is described in U.S. Pat. No. 5,451,569, issued Sep. 19,1995 to Wong et al.

Contemplated for use in the practice of this invention are a wide rangeof mechanical devices designed for pulmonary delivery of therapeuticproducts, including but not limited to nebulizers, metered doseinhalers, and powder inhalers, all of which are familiar to thoseskilled in the art. With regard to construction of the delivery device,any form of aerosolization known in the art, including but not limitedto spray bottles, nebulization, atomization or pump aerosolization of aliquid formulation, and aerosolization of a dry powder formulation, canbe used in the practice of the invention.

Some specific examples of commercially available devices suitable forthe practice of this invention are the Ultravent nebulizer, manufacturedby Mallinckrodt, Inc., St. Louis, Mo.; the Acorn II nebulizer,manufactured by Marquest Medical Products, Englewood, Colo.; theVentolin metered dose inhaler, manufactured by Glaxo Inc., ResearchTriangle Park, North Carolina; and the Spinhaler powder inhaler,manufactured by Fisons Corp., Bedford, Mass.

All such devices require the use of formulations suitable for thedispensing of pharmaceutical composition of the present invention.Typically, each formulation is specific to the type of device employedand may involve the use of an appropriate propellant material, inaddition to the usual diluents, adjuvants and/or other carriers usefulin therapy. Also, the use of liposomes, microcapsules or microspheres,inclusion complexes, or other types of carriers is contemplated.Chemically modified pharmaceutical composition of the present inventionmay also be prepared in different formulations depending on the type ofchemical modification or the type of device employed.

Formulations suitable for use with a nebulizer, either jet orultrasonic, will typically comprise a compound or pharmaceuticalcomposition of the present invention dissolved in water at aconcentration of about 0.1 to 25 mg of biologically active ingredientsper mL of solution. The formulation may also include a buffer and asimple sugar (e.g., for stabilization and regulation of osmotic pressureof a pharmaceutical composition of the present invention). The nebulizerformulation may also contain a surfactant, to reduce or prevent surfaceinduced aggregation of a compound or pharmaceutical composition of thepresent invention caused by atomization of the solution in forming theaerosol.

Formulations for use with a metered-dose inhaler device will generally,for example, comprise a finely divided powder containing a compound orpharmaceutical composition of the present invention suspended in apropellant with the aid of a surfactant. The propellant may be anysuitable material employed for this purpose, such as achlorofluorocarbon, a hydrochlorofluorocarbon, a hydrofluorocarbon, or ahydrocarbon, including trichlorofluoromethane, dichlorodifluoromethane,dichlorotetrafluoroethanol, and 1,1,1,2-tetrafluoroethane, orcombinations thereof. Suitable surfactants include sorbitan trioleateand soya lecithin. Oleic acid may also be useful as a surfactant.

The liquid aerosol formulations contain a compound or pharmaceuticalcomposition of the present invention and a dispersing agent in aphysiologically acceptable diluent. The dry powder aerosol formulationsof the present invention can contain a finely divided solid form of acompound or pharmaceutical composition of the present invention and adispersing agent. With either the liquid or dry powder aerosolformulation, the formulation must be aerosolized. That is, it must bebroken down into liquid or solid particles in order to ensure that theaerosolized dose actually reaches the mucous membranes of the nasalpassages or the lung. The term “aerosol particle” is used herein todescribe the liquid or solid particle suitable for nasal or pulmonaryadministration, i.e., that will reach the mucous membranes. Otherconsiderations include the construction of the delivery device,additional components in the formulation, and particle characteristics.These aspects of nasal or pulmonary administration of a drug are wellknown in the art, and manipulation of formulations, aerosolization meansand construction of a delivery device can be readily achieved by one ofordinary skill in the art.

In a particular embodiment, the mass median dynamic diameter will be 5micrometers or less in order to ensure that the drug particles reach thelung alveoli (Wearley, L. L., 1991, 1991, Crit. Rev. in Ther. DrugCarrier Systems 8:333).

As noted above, in a particular aspect of the invention, the device foraerosolization is a metered dose inhaler. A metered dose inhalerprovides a specific dosage when administered, rather than a variabledose depending on administration. Such a metered dose inhaler can beused with either a liquid or a dry powder aerosol formulation. Metereddose inhalers are well known in the art.

Often, the aerosolization of a liquid or a dry powder formulation forinhalation into the lung will require a propellant. The propellant maybe any propellant generally used in the art. Specific nonlimitingexamples of such useful propellants are a chloroflourocarbon, ahydrofluorocarbon, a hydrochlorofluorocarbon, or a hydrocarbon,including triflouromethane, dichlorodiflouromethane,dichlorotetrafluoroethanol, and 1,1,1,2-tetraflouroethane, orcombinations thereof.

Systems of aerosol delivery, such as the pressurized metered doseinhaler and the dry powder inhaler are disclosed in Newman, S. P.,Aerosols and the Lung, Clarke, S. W. and Davia, D. editors, pp. 197-22and can be used in connection with the present invention.

Liquid Aerosol Formulations

The present invention provides for liquid aerosol formulations anddosage forms of a compound or pharmaceutical composition of the presentinvention. In general, such dosage forms contain a compound orpharmaceutical composition of the present invention in apharmaceutically acceptable diluent. Pharmaceutically acceptablediluents include, but are not limited to sterile water, saline, bufferedsaline, dextrose solution, and the like. In a specific embodiment, adiluent that may be used in the present invention or the pharmaceuticalformulation of the present invention is phosphate buffered saline, or abuffered saline solution generally between the pH 7.0-8.0 range, orwater.

The liquid aerosol formulation of the present invention may include, asoptional ingredients, pharmaceutically acceptable carriers, diluents,solubilizing or emulsifying agents, surfactants and excipients.

The formulations of the present embodiment may also include other agentsuseful for pH maintenance, solution stabilization, or for the regulationof osmotic pressure. Examples of the agents include, but are not limitedto salts, such as sodium chloride, or potassium chloride, andcarbohydrates, such as glucose, galactose or mannose, and the like.

Aerosol Dry Powder Formulations

It is also contemplated that the present aerosol formulation can beprepared as a dry powder formulation comprising a finely divided powderform of a compound or pharmaceutical composition of the presentinvention and a dispersant.

Formulations for dispensing from a powder inhaler device can comprise afinely divided dry powder containing a compound or pharmaceuticalcomposition of the present invention, and may also include a bulkingagent, such as lactose, sorbitol sucrose, or mannitol in amounts whichfacilitate dispersal of the powder from the device, e.g., 50 to 90% byweight of the formulation. A compound or pharmaceutical composition ofthe present invention should most advantageously be prepared inparticulate form with an average particle size of less than 10 microns,most preferably 0.5 to 5 microns, for most effective delivery to thedistal lung.

In another embodiment, the dry powder formulation can comprise a finelydivided dry powder containing a compound or pharmaceutical compositionof the present invention, a dispersing agent and also a bulking agent.Bulking agents useful in conjunction with the present formulationinclude such agents as lactose, sorbitol, sucrose, or mannitol, inamounts that facilitate the dispersal of the powder from the device.

Transdermal Administration

Various and numerous methods are known in the art for transdermaladministration of a drug, e.g., via a transdermal patch. Transdermalpatches are described in for example, U.S. Pat. No. 5,407,713, issuedApr. 18, 1995 to Rolando et al.; U.S. Pat. No. 5,352,456, issued Oct. 4,1994 to Fallon et al.; U.S. Pat. No. 5,332,213 issued Aug. 9, 1994 toD'Angelo et al.; U.S. Pat. No. 5,336,168, issued Aug. 9, 1994 toSibalis; U.S. Pat. No. 5,290,561, issued Mar. 1, 1994 to Farhadieh etal.; U.S. Pat. No. 5,254,346, issued Oct. 19, 1993 to Tucker et al.;U.S. Pat. No. 5,164,189, issued Nov. 17, 1992 to Berger et al.; U.S.Pat. No. 5,163,899, issued Nov. 17, 1992 to Sibalis; U.S. Pat. Nos.5,088,977 and 5,087,240, both issued Feb. 18, 1992 to Sibalis; U.S. Pat.No. 5,008,110, issued Apr. 16, 1991 to Benecke et al.; and U.S. Pat. No.4,921,475, issued May 1, 1990 to Sibalis, the disclosure of each ofwhich is incorporated herein by reference in its entirety.

It can be readily appreciated that a transdermal route of administrationmay be enhanced by use of a dermal penetration enhancer, e.g., such asenhancers described in U.S. Pat. No. 5,164,189 (supra), U.S. Pat. No.5,008,110 (supra), and U.S. Pat. No. 4,879,119, issued Nov. 7, 1989 toAruga et al., the disclosure of each of which is incorporated herein byreference in its entirety.

Moreover, a compound or pharmaceutical composition of the presentinvention can be administered topically. For example, a compound can bemixed with a salve carrier forming a composition that can be rubbed ontothe skin. Alternatively, a compound of the present invention can bedissolved into a solvent that is well known to permeate the skin. Aparticular example of such a solvent is dimethyl sulfoxide (DMSO). Gelformulations are also contemplated.

Oral Delivery

Contemplated for use herein are oral solid dosage forms, which aredescribed generally in Remington's Pharmaceutical Sciences, 18th Ed.1990 (Mack Publishing Co. Easton Pa. 18042) at Chapter 89, which isherein incorporated by reference. Solid dosage forms include, forexample, tablets, capsules, pills, troches or lozenges, cachets orpellets. Also, liposomal or proteinoid encapsulation may be used toformulate a compound or pharmaceutical composition of the presentinvention (as, for example, proteinoid microspheres reported in U.S.Pat. No. 4,925,673). Liposomal encapsulation can be used and theliposomes can be derivatized with various polymers (e.g., U.S. Pat. No.5,013,556). A description of possible solid dosage forms for therapeuticuse is given by Marshall K. In: Modern Pharmaceutics Edited by G. S.Banker and C. T. Rhodes Chapter 10, 1979, herein incorporated byreference. The formulation can include a compound or pharmaceuticalcomposition of the present invention and inert ingredients which allowfor protection against the stomach environment, and release of thebiologically active material in the intestine.

Also specifically contemplated are oral dosage forms of a compound ofthe present invention, wherein the compound may be chemically modifiedso that oral delivery of the derivative is efficacious. Generally, thechemical modification contemplated is the attachment of at least onemoiety to the component molecule itself; where the moiety permits (a)inhibition of proteolysis; and (b) uptake into the blood stream from thestomach or intestine. Also desired is the increase in overall stabilityof a compound of the present invention and increase in circulation timein the body. Examples of such moieties include: polyethylene glycol,copolymers of ethylene glycol and propylene glycol, carboxymethylcellulose, dextran polyvinyl alcohol, polyvinyl pyrrolidone andpolyproline. Abuchowski and Davis, 1981, “Soluble Polymer-EnzymeAdducts” In: Enzymes as Drugs, Hocenberg and Roberts, eds.,Wiley-Interscience, New York, N.Y., pp. 367-383; Newmark, et al., 1982,J. Appl. Biochem. 4:185-189. Other polymers that could be used arepoly-1,3-dioxolane and poly-1,3,6-tioxocane. Preferred forpharmaceutical usage, as indicated above, are polyethylene glycolmoieties.

For a compound of the present invention, the location of release may bethe stomach, the small intestine (the duodenum, the jejunum, or theileum), or the large intestine. One skilled in the art can prepareformulations which will not dissolve in the stomach, yet will release acompound of the present invention in the duodenum or elsewhere in theintestine. Preferably, the release will avoid the deleterious effects ofthe stomach environment, either by protection of the compound or byrelease of the biologically active material beyond the stomachenvironment, such as in the intestine.

To ensure full gastric resistance, a coating impermeable to at least pH5.0 is essential. Examples of the more common inert ingredients that areused as enteric coatings are cellulose acetate trimellitate (CAT),hydroxypropylmethylcellulose phthalate (HPMCP), HPMCP 50, HPMCP 55,polyvinyl acetate phthalate (PVAP), Eudragit L30D, Aquateric, celluloseacetate phthalate (CAP), Eudragit L, Eudragit S, and Shellac. Thesecoatings may be used as mixed films.

A coating or mixture of coatings can also be used on tablets, which arenot intended for protection against the stomach. This can include sugarcoatings, or coatings which make the tablet easier to swallow. Capsulesmay consist of a hard shell (such as gelatin) for delivery of drytherapeutic i.e. powder; for liquid forms, a soft gelatin shell may beused. The shell material of cachets could be thick starch or otheredible paper. For pills, lozenges, molded tablets or tablet triturates,moist massing techniques can be used.

A compound of the present invention can, for example, be included in theformulation as fine multi-particulates in the form of granules orpellets of particle size about 1 mm. The formulation of the material forcapsule administration could also be as a powder, lightly compressedplugs or even as tablets. Also, a compound or pharmaceutical compositionof the present invention can be prepared by compression.

Colorants and flavoring agents may all be included. For example, thecompound can be formulated (such as by liposome or microsphereencapsulation) and then further contained within an edible product, suchas a refrigerated beverage containing colorants and flavoring agents.

One may dilute or increase the volume of a compound of the presentinvention or a pharmaceutical composition with an inert material. Thesediluents could include carbohydrates, especially mannitol a-lactose,anhydrous lactose, cellulose, sucrose, modified dextrans and starch.Certain inorganic salts may be also be used as fillers including calciumtriphosphate, magnesium carbonate and sodium chloride. Some commerciallyavailable diluents are Fast-Flo, Emdex, STA-Rx 1500, Emcompress andAvicell.

Disintegrants may be included in the formulation of a pharmaceuticalcomposition of the present invention into a solid dosage form. Materialsused as disintegrates include, but are not limited to starch, includingthe commercial disintegrant based on starch, Explotab. Sodium starchglycolate, Amberlite, sodium carboxymethylcellulose, ultramylopectin,sodium alginate, gelatin, orange peel, acid carboxymethyl cellulose,natural sponge and bentonite may all be used. Another form of thedisintegrants are the insoluble cationic exchange resins. Powdered gumsmay be used as disintegrants and as binders and these can includepowdered gums such as agar, Karaya or tragacanth. Alginic acid and itssodium salt are also useful as disintegrants.

Binders may be used to hold a compound or pharmaceutical composition ofthe present invention together to form a hard tablet and includematerials from natural products such as acacia, tragacanth, starch andgelatin Others include methyl cellulose (MC), ethyl cellulose (EC) andcarboxymethyl cellulose (CMC). Polyvinyl pyrrolidone (PVP) andhydroxypropylmethyl cellulose (HPMC) could both be used in alcoholicsolutions to granulate the therapeutic.

An anti-frictional agent may be included in the formulation of apharmaceutical composition of the present invention to prevent stickingduring the formulation process. Lubricants may be used as a layerbetween the therapeutic and the die wall, and these can include, but arenot limited to; stearic acid including its magnesium and calcium salts,polytetrafluoroethylene (PTFE), liquid paraffin, vegetable oils andwaxes. Soluble lubricants may also be used such as sodium laurylsulfate, magnesium lauryl sulfate, polyethylene glycol of variousmolecular weights, Carbowax 4000 and 6000.

Glidants that might improve the flow properties of a compound of thepresent invention during formulation and to aid rearrangement duringcompression can be added. The glidants may include starch, talc,pyrogenic silica and hydrated silicoaluminate.

Additives which potentially enhance uptake of a compound of the presentinvention administered orally are, for instance, the fatty acids oleicacid, linoleic acid and linolenic acid.

Controlled release oral formulation may be desirable. The drug could beincorporated into an inert matrix which permits release by eitherdiffusion or leaching mechanisms, e.g., gums. Slowly degeneratingmatrices may also be incorporated into the formulation. Some entericcoatings also have a delayed release effect.

Another form of a controlled release of a compound of the presentinvention is by a method based on the Oros therapeutic system (AlzaCorp.), i.e. the drug is enclosed in a semipermeable membrane whichallows water to enter and push drug out through a single small due toosmotic effects.

Other coatings may be used for the formulation. These include a varietyof sugars which could be applied in a coating pan. A compound of thepresent invention could also be given in a film coated tablet and thematerials used in this instance can be, for example, divided into 2groups. The first are the nonenteric materials and include methylcellulose, ethyl cellulose, hydroxyethyl cellulose, methylhydroxy-ethylcellulose, hydroxypropyl cellulose, hydroxypropyl-methyl cellulose,sodium carboxy-methyl cellulose, providone and the polyethylene glycols.The second group includes the enteric materials that are commonly estersof phthalic acid

A mix of materials might be used to provide the optimum film coating.Film coating may be carried out in a pan-coater or in a fluidized bed orby compression coating.

Methods for Preparation

Compounds of the formula I described above can be prepared by anysuitable method, for example, by contacting the desired compound Q(especially, organic amine, amino acid or amino acid ester) with thefree acid of combretastatin A-4 phosphate (“CA4P free acid”) which hasthe structure:

in relative amounts sufficient to obtain a mono- or di-organic aminesalt, mono- or di-amino acid salt or mono- or di-amino acid ester saltof formula I of the present invention (e.g., 1:1 molar ratio to obtain a1:1 mono-organic amine salt or mono-amino acid salt, or a molar excessof organic amine in an appropriate solvent (e.g., a solvent selected fora desirable pKa) to obtain a di-organic amine salt). CA4P free acid canbe obtained from CA4P disodium salt, for example, as described in theExamples infra. The compound Q, such as organic amine or amino acid, andCA4P free acid can, for example, be contacted in a suitable solvent(preferably, a C₁-C₆ alcohol such as isopropanol or aqueous mixturesthereof), preferably followed by collection of the compound of formula Ias a crystalline compound by means such as filtration. The term“solvent” includes a single solvent, or mixtures of two or more solventsforming miscible or biphasic solvent mixtures. Where desired, thecompound Q can be added in the form of a salt, preferably apharmaceutically acceptable salt, as described in the Examples infra.

Thus, the present invention extends to a process for preparing acompound having a general structure of the formula I:

where one of —OR¹ or —OR² is —O—QH⁺, and the other is hydroxyl or—O—QH⁺, and Q is(A) an organic amine containing at least one nitrogen which, togetherwith a proton, forms a quaternary cation QH⁺;(B) an amino acid containing at least two nitrogen atoms where one ofthe nitrogen atoms, together with a proton, forms a quaternary ammoniumcation QH⁺; or(C) an amino acid containing one or more nitrogen atoms where one of thenitrogen atoms, together with a proton, forms a quaternary ammoniumcation QH⁺ and where, further, all carboxylic acid groups of the aminoacid are in the form of esters.

Such a method of the present invention comprises the step of contacting,in a solvent, CA4P free acid having the structure:

with a compound Q, where Q is(A) an organic amine containing at least one nitrogen atom which iscapable of forming, together with a proton, a quaternary ammonium cationQH⁺;(B) an amino acid containing at least two nitrogen atoms where one ofthe nitrogen atoms, together with a proton, forms a quaternary ammoniumcation QH⁺; or(C) an amino acid containing one or more nitrogen atoms where one of thenitrogen atoms, together with a proton, forms a quaternary ammoniumcation QH⁺ and where, further, all carboxylic acid groups of the aminoacid are in the form of esters.

Optionally, a compound of the present invention produced with a processdescribed herein can be precipitated in crystalline form from thesolvent.

In addition, the present invention extends to a process for preparing acompound having a general structure of the formula I:

as described above, comprising the steps of contacting the CA4P freeacid with a preferred compound Q (such as histidine, a glycine C₁₋₆alkyl ester or, most preferably, tromethamine) in the solvent, and thencollecting the resulting CA4P histidine, glycine C₁₋₆ alkyl ester ormost preferably, tromethamine salt in crystalline form from the solvent.Naturally, as explained above, numerous solvents have applications in aprocess of the present invention. Particular examples, include, butcertainly are not limited to C₁-C₆ alcohols, such as isopropanol oraqueous mixtures thereof. In a preferred embodiment of the presentinvention, a process described herein produces the compound CA4 Pmono-tromethamine (“mono-TRIS salt of CA4P” or “CA4P mono TRIS salt”).In another preferred embodiment of the present invention a processdescribed herein produces the compound CA4P mono-L-histidine.

Mixtures of a compound Q (organic amine, amino acid or amino acid ester)and CA4P free acid, preferably in a solution such as an aqueoussolution, are also contemplated herein as embodiments of the invention.Thus, the present invention further provides compositions formed bymixing compounds comprising:

(a) CA4P free acid having the structure of:

(b) a compound Q, wherein Q is(A) an organic amine containing at least one nitrogen atom which iscapable of forming, together with a proton, a quaternary ammonium cationQH⁺;(B) an amino acid containing at least two nitrogen atoms where one ofthe nitrogen atoms, together with a proton, forms a quaternary ammoniumcation QH⁺; or(C) an amino acid containing one or more nitrogen atoms where one of thenitrogen atoms, together with a proton, forms a quaternary ammoniumcation QH⁺ and where, further, all carboxylic acid groups of the aminoacid are in the form of esters.

Optionally, a composition of the present invention can further comprisea pharmaceutically acceptable carrier.

The present invention may be better understood by reference to thefollowing non-limiting Examples, which are provided as exemplary of theinvention. The following Examples are presented in order to more fullyillustrate the preferred embodiments of the invention. They should in noway be construed, however, as limiting the broad scope of the invention.

Example 1 CA4P Prodrug Mono-Tromethamine Salt

In an embodiment of the present invention, a CA4P mono-tromethanine saltis described as a non-limiting example of a compound of the presentinvention. With the information described herein, one of ordinary skillin the art can readily produce a variety of CA4P prodrug mono- ordi-organic amine salts, such as by addition of the desired organic amineto CA4P free acid by a procedure analogous to that described following,all of which are encompassed by the present invention and the appendedclaims.

Reagents and Methods

All reagents and chemicals were obtained from commercial sources andused without further purification: Tris(hydroxymethyl)aminomethane(TRIS) (Aldrich Chemical Co. 99.9+% labeled, Lot #01404PU), isopropylalcohol (B&J Brand, High purity solvent grade). Multi-nuclei NMR spectrawere recorded on Bruker DRX 400 spectrometer. The ¹H and ¹³C NMRchemical shifts are reported in ppm relative to tetramethylsilane. (The¹³C NMR chemical shifts were determined using methanol (“MeOH”) asexternal standard). The ¹³C NMR spectra were acquired with protondecoupled {¹H}. The 2D NMR experiments (HMQC (Heteronuclear MultipleQuantum Correlation spectroscopy, an inverse chemical shift correlationexperiment to determine which ¹H's of the molecule are bonded to which¹³C nuclei (or other X nuclei)); and HMQC (Heteronuclear Multiple BondCorrelation spectroscopy, a modified version of HMQC suitable todetermine long-range ¹H-¹³C connectivity, as well as the structure and¹H and ¹³C assignments of the molecule)) were conducted to aid theassignments of the ¹H and ¹³C NMR signals to the structure. “CA4Pdisodium salt” is the compound having the following structure:

(See U.S. Pat. No. 5,561,122 mentioned above).CA4P Mono-Tromethamine Salt

Aqueous IPA TRIS solution (0.19 M). A 0.19 M TRIS solution was preparedby dissolving 1.61 g of TRIS (13.3 mmol) in 7 mL of deionized water, andadded 63 mL of isopropyl alcohol (“IPA”) to the resulting aqueoussolution (10% water in IPA).

CA4P free acid stock solution in isopropyl alcohol (0.19M). CA4Pdisodium salt (12.15 g, 27.6 mmol) was dissolved in 70 mL of deionizedwater. Ethyl acetate (250 mL) and a saturated aqueous sodium chloridesolution (150 mL) were added to the resulting solution with rapidstirring. A white cake was formed. A solution of 0.5 N hydrochloric acid(325 mL) was added portion-wise to dissolve the cake (the final pH ofthe aqueous phase was ca. 1 (pH paper)). The organic phase wasseparated. The aqueous phase was extracted with ethyl acetate (3×200mL). The organic phases were combined and dried over anhydrous Na₂SO₄.Filtration and solvent evaporation (Rotavapor, water bathtemperature=40° C.) yielded a thick film of CA4P free acid which wasdissolved in 100 mL of IPA. The concentration of the resulting solutionwas determined to be 0.19 M by ¹H NMR, using the following titrationmethod: pipetted 45 μL of L-histidine solution (0.17 M) and 30 μL ofCA4P free acid solution into a 4-mL HPLC vial. The solvents wereevaporated to dryness using the Rotavapor. The solid was dissolved in0.7 mL of D₂O and analyzed by ¹H NMR to give a 1:0.75 ratio of histidineto CA4P free acid. A total of 19 mmol of CA4P free acid was obtained(69% yield).

CA4P mono-TRIS salt. A 200-mL round bottom flask was charged with 70 mLof the CA4P flee acid solution prepared as above (0.19 M, 13.3 mmol). 70mL of the aqueous IPA TRIS solution prepared as above (0.19 M, 13.3mmol) was added portion-wise to the CA4P free acid solution with rapidstirring. The resulting white slurry was stirred at ambient temperaturefor 18 hours (overnight), followed by cooling to 0° C. (ice-bath) for 30min. The crystalline solid was isolated by filtration through a Whatman#54 filter paper with suction, washed with cold isopropyl alcohol, anddried in a stream of air for 5 hours and then in vacuo (vacuumdesiccator) for 113 hours to yield 7.01 g of CA4P mono-TRIS salt as awhite solid. The results of the ¹H NMR analysis showed that the finalproduct contained IPA (ca. 0.9 wt %) as a residual solvent (13.4 mmol,quantitative yield). The CA4P mono-TRIS salt has the structure offormula Ia, where the olefin group bridging the phenyl moieties of thecore is in the cis configuration as in the combretastatin A-4 phosphatestaring material.

Characterization of CA4P mono TRIS Salt

NMR and Elemental Analysis

¹H NMR (400 MHz, D₂O) δ 3.52 (s, 6H, C(15)H₃ and C(17)H₃), 3.56 (s, 6H,C(20)H₂, C(21)H₂, C(22)H₂), 3.59 (s, 3H, C(16)H₃), 3.67 (s, 3H,C(18)H₃), 6.38 (d, J=11.7 Hz, 1H, C(8)H), 6.46 (d, J=11.7 Hz, 1H,C(7)H), 6.48 (s, 2H, C(10)H and C(14)H, 6.79 (d, J=8.8 Hz, 1H, C(3)H),6.85 (broad d, J=8.8 Hz, 1H, C(4)H), 7.06 (broad s, 1H, C(6)H); ¹³C NMR(100 MHz, {¹H}, D₂O) δ 56.9 (2C, C15, C17), 57.0 (C18), 60.3 (3C, C20,C21, C22), 62.0 (C16), 62.4 (C19), 107.5 (2C, C10, C14), 113.9 (C3),122.6 (d, J_(PC)=3.1 Hz, C6), 125.9 (C4), 130.0 (C8), 130.8 (C7), 1312(CS), 134.7 (C9), 136.8 (C12), 142.2 (d, J_(PC)=7.7 Hz, C1), 150.6 (d,J_(PC)=6.1 Hz, C2), 153.2 (2C, C11 and C13). Anal. Calcd forC₂₂H₃₂NO₁₁P: C, 51.06; H 6.23; N, 2.70; P, 5.98. Found. C, 51.07; H,6.39; N, 2.58; P, 5.93.

Hygroscopicity

The mono TRIS salt of CA4P of this Example was found to be practicallynonhygroscopic at 25° C. under ambient and high humidity conditions.This was an unexpected result, because other salt forms of CA4P freeacid are hygroscopic under similar conditions. The moisture sorptionprofile of the mono TRIS salt of CA4P is shown in FIG. 1. Variablerelative humidity-X-ray diffraction (“RH-XRD”) experiments have shownthat the powder pattern remains unchanged on exposure to differenthumidities at 25° C.

Polymorphism

Single crystal X-ray studies on the CA4P mono TRIS salt of this Exampledemonstrate that it is an achiral neat form (N−1), which does notcontain any solvent sites, and that it has a centrosymmetric monocliniccrystal structure. The simulated powder pattern, which was derived fromthe refined atomic parameters in the monoclinic single crystal structureat room temperature, is consistent with the observed powder pattern.Several lots of the mono TRIS salt prepared from IPA/water were found tobe reproducible based on ¹H NMR, differential scanning calorimetry(DSC), thermogravimetry (TGA) and powder X-ray diffraction (p-XRD). TheCA4P mono TRIS salt was slurried in several different solvents such asethanol, isopropanol acetone, acetonitrile and water, initially at70-75° C. for 5-10 min, and then at room temperature overnight. Theresulting solid phases were analyzed by DSC, TGA and p-XRD. None of theslurried samples showed the presence of any solvates in either theslurry or dry states. There were no differences in the DSC thermogramsand the p-XRD patterns (see FIG. 2) in any of these samples as comparedto the “as is” material. This indicates that this N−1 form is arelatively stable, single polymorph form.

Other Physicochemical Properties

The DSC thermogram of CA4P mono TRIS salt shows a single melt endothermat 196° C. (FIG. 3). Thermogravimetric analysis did not reveal anyweight losses below 150° C. due to volatilization. The equilibriumaqueous solubility of the CA4P mono TRIS salt at 25° C. was determinedto be 3.37 mg/mL (pH 4.8). The aqueous solubility increases with anincrease in pH and attains a value of 191 mg/mL at pH 8.2. This isespecially suitable for preparing a dosage form of the compound of thisExample in the pH range 8-9 (including, but not limited to solutions tobe directly administered to a patient (“Ready-to-Use Solutions”), orbatching solutions for lyophilization) for intravenous administration.The pH-solubility profile of CA4P mono TRIS salt is shown in FIG. 4. Themono TRIS salt also exhibited good chemical stability in the solid-stateon exposure to ambient and accelerated conditions of temperature andhumidity.

The mono TRIS salt of CA4P of this Example thus has excellentphysicochemical properties for use in a pharmaceutical formulation, forexample, intended for either oral or parenteral administration. Unlikeother salt forms of CA4P not contemplated herein, the mono TRIS saltshowed unexpectedly superior properties in the solid-state, particularlythe practically nonhygroscopic behavior. This was especially surprisingin view of the degree of water solubility of TRIS per se.

CA4P Mono-Tromethamine Salt (Scale-Up)

CA4P free acid solution in IPA. A 12-L three-neck round-bottom flask wasequipped with a mechanical stirrer and an additional funnel. A solutionof CA4P disodium salt (99.92 g, 0.227 mol) in 1.5 L of deionized waterand ethyl acetate (2.0 L) were added to the flask. A solution ofhydrochloric acid (0.5 N, 950 mL, 0.475 mol) was added slowly via theadditional funnel with rapid stirring (the final pH of the aqueous phasewas ca. 1 (pH paper)). The organic phase was separated. The aqueousphase was extracted with ethyl acetate (5×1.6 L). The combined organicphases were dried over Na₂SO₄. The ethyl acetate was rotary evaporatedto form a thick oil which was dissolved in IPA (800 mL).

CA4P mono-TRIS salt. A solution of TRIS (25.0 g, 0.206 mol) in 800 mL ofdeionized water was charged to a 12-L three-neck round-bottom flask.CA4P free acid solution in IPA prepared above was added slowly via anadditional funnel with rapid stirring. After addition, the resultingsolution was seeded with CA4P mono-TRIS salt and mechanically stirred atRT for 1 h. Then, more IPA (2.0 L) was slowly added to the slurry andstirring was continued for another 1 h. The crystalline white solid wasisolated by filtration with suction, washed with IPA (800 mL), and driedin a vacuum oven at about 40° C. for 4 days to give 101.55 g of CA4Pmono-TRIS salt. The results of the ¹H NMR analysis of the final productshowed that it contained IPA (0.4 wt %) as a residual solvent (0.196mol, 86% overall yield). Anal. Calcd for C₂₂H₃₂NO₁₁P: C, 51.06; H 6.23;N, 2.70; P, 5.98. Found: C, 50.95; H, 6.14; N, 2.69; P, 5.82.

Example 2 CA4P Prodrug Mono-Tromethamine Salt TRIS Formulation

Aqueous solution and lyophile (i.e., freeze dried) pharmaceuticalcompositions of the compound of Example 1 (CA4P mono TRIS salt) wereprepared as follows.

An aqueous solution of the compound of Example 1 was obtained bydissolving the compound in Water for Injection, USP to a concentrationof 60 mg/mL with the addition of a sufficient amount of TRIS(tromethamine base) to obtain a pH of 8.5. The dissolution was carriedout under the protection from light.

A lyophile was then obtained by the following procedure. The solutionformed above was filtered through a suitable 0.2 micron sterilizingfilter and aliquoted into sterile glass vials. The solution waslyophilized in a Virtis Lyophilizer at −35° C. under high vacuum over aperiod of 24 to 72 hours and then further dried at 5° C. under highvacuum for 24 to 48 hours to yield a lyophile.

Other pharmaceutical compositions containing the compound of Example 1can be prepared in accordance with these procedures. For example, asdescribed above, bulking agents (e.g., amino acids such as arginine, orlysine, sugars such as sucrose, lactose, mannitol, polymers such aspolyvinylpyrrolidones or dextran, etc.) or other excipients can be addedto the above solution.

For example, an aqueous solution of the compound of Example 1 wasobtained by dissolving the compound in Water for Injection, USP to aconcentration of 30 mg/mL with the addition of a suitable bulking agentsuch as mannitol, dextran, or combinations thereof to a concentration of100 mg/mL and a sufficient amount of TRIS (tromethamine base) to obtaina pH of 8.6. The dissolution was carried out under the protection fromlight.

A lyophile was then obtained by the following procedure. The solutionformed above was filtered through a suitable 0.2 micron sterilizingfilter and aliquoted into sterile glass vials. The solution waslyophilized in a Virtis Lyophilizer at −10° C. under moderate vacuumover a period of 24 to 72 hours and then further dried at 5° C. underhigh vacuum for 24 to 48 hours to yield a lyophile.

As explained above, any suitable organic amines including, but notlimited to diethanolamine, glucamine, N-methylglucamine,ethylenediamine, and 2-4-imidazolyl) ethyl amine, can readily besubstituted for tromethamine in the Examples described above to producea compound or composition of the present invention. Compositions whereinQ has other definitions within formula I can be similarly formed.

Example 3 CA4P Mono-L-Histidine Salt Prodrug

In an embodiment of the present invention, a CA4P mono-L-histidine saltis described as a non-limiting example of a compound of the presentinvention. With the information described herein, one of ordinary skillin the art can readily produce a variety of CA4P mono- or di-amino acidsalt prodrugs, such as by addition of the desired amino acid to CA4Pfree acid by a procedure analogous to that described following, all ofwhich are encompassed by the present invention and the appended claims.

Reagents and Methods

The following reagents and chemicals were obtained from commercialsources and used without further purification: L-Histidine (AldrichChemical Co. 98% labeled, Lot #04821JR), methanol and isopropyl alcohol(B&J Brand, High purity solvent grade). “CA4P disodium salt” is thecompound having the following structure:

(See U.S. Pat. No. 5,561,122 mentioned above).

Multi-nuclei NMR spectra were recorded on Bruker DPX 300 and DRX 400spectrometers. The ¹H and ¹³C NMR chemical shifts are reported in ppmrelative to tetramethylsilane (the ¹³C NMR chemical shifts weredetermined using methanol as external standard). The ³¹P NMR chemicalshifts are reported in ppm relative to 85% H₃PO₄ (external standard).The ¹³C and ³¹P NMR spectra were acquired with proton decoupled {¹H}.The 2D NMR experiments (HMQC and HMBC) were conducted to aid theassignments of the ¹H and ¹³C NMR signals to the structure. DSC wereperformed on DSC/2920 Differential Scanning Calorimeter, TA Instruments.

CA4P Mono-L-Histidine Hydrated Salt

L-Histidine aqueous stock solution (0.2 M). L-Histidine (0.3167 g, 2.0mmol) was dissolved in 10 mL of deionized water to form a 0.2 Msolution.

CA4P free acid stock solution in methanol (0.6 M). CA4P disodium salt(1.9194 g) was dissolved in 5.0 mL of deionized water. A sodium chloridesaturated aqueous solution (40 mL) was added to the resulting solution.White solid precipitated Ethyl acetate (50 mL) was added and theresulting slurry was magnetically stirred and acidified with a solutionof 0.5 N hydrochloric acid until the biphasic mixture became clear (theaqueous phase was acidic (pH paper)). The organic phase was separated.The aqueous phase was extracted with ethyl acetate (2×50 mL). Theorganic phases were combined and dried over Na₂SO₄. Filtration andsolvent evaporation (rotavapor, water bath temperature=37° C.) yielded athick film of CA4P free acid which was taken up with 10 mL of methanol.The methanol was rotary evaporated (37° C.) to give an off-white foam(152 g), which was re-dissolved in MeOH (4.79 mL) to yield a solutionwith an expected 0.8 M concentration.

The concentration determined above was further confirmed by mixing 100μL of a 0.2 M histidine solution (20 μmol) with 25 μL of CA4P free acidsolution. The solvents of the resulting solution were rotary evaporatedto dryness. The solid was analyzed by ¹H NMR to show a 1:0.75 mole ratioof histidine: CA4P free acid. Therefore, the concentration of CA4P freeacid was figured out as 0.6 M. (Thus result indicated that the foam ofCA4P free acid contained solvent).

CA4P mono-L-histidine hydrated salt. To a 4-mL HPLC vial were addedL-histidine (900 μL, 0.2 M, 180 μmol), CA4P free acid (300 μL, 0.6 M,180 μL), and isopropyl alcohol (1.0 mL). The resulting solution wasrotary evaporated (water bath temperature=39-40° C.) to reduce thevolume down to ca 1.5 mL. Another 1.0 mL of isopropyl alcohol was addedand the volume was further reduced to ca. 15 mL. A small crystal wasobserved in the solution. The evaporation process was stopped and thevial was capped and allowed to stand at ambient temperature for 5 h. Thecrystalline solid was isolated by filtration through a Whatman #54filter paper with suction, washed with isopropyl alcohol (ca. 2 mL), anddried in a stream of nitrogen overnight to yield 82.7 mg of CA4Pmono-L-histidine as a white solid. The CA4P mono-L-histidine saltprodrug obtained was a crystalline solid; Karl Fisher analysis of thesolid showed that the water content was 4.66%, which calculated to acrystalline solid hydrated with 1.5 water molecules per salt molecule(143 μmol, 79% yield): ¹H NMR (300 MHz, D₂O) δ 3.33 (d, J=6.59 Hz, 2H,C(21)H₂), 3.67 (s, 6H, C(15)H₃ and C(17)H₃), 3.74 (s, 3H, C(16)H₃), 3.82(s, 3H, C(18)H₃), 4.01 (t, J=6.50 Hz, 1H, C(20)H), 6.53 (d, J=12.25 Hz,1H, C(8)H, 6.62 (d, J=12.25 Hz, 1H, C(7)H), 6.62 (s, 2H, C(10)H andC(14)H), 6.94 (d, J=8.00 Hz, 1H, C(3)H), 7.01 (d, J=8.00 Hz, 1H, C(4)H),7.21 (s, 1H, C(6)H), 7.37 (s, 1H, C(23)H, 8.63 (s, 1H, C(24)H; ¹³C NMR(100 MHz, {¹H}, D₂O) δ 26.12 (C21), 53.93 (C20), 56.26 (2C, C15, C17),56.35 (C18), 61.32 (C16), 106.86 (2C, C10, C14), 113.26 (C3), 118.03(C23), 122.04 (d, J_(PC)=2.3 Hz, C6), 125.52 (C4), 127.80 (C22), 129.40(C8), 130.05 (C7), 130.57 (C5), 133.99 (C9), 134.34 (C24), 136.18 (C12),141.37 (d, J_(PC)=6.90 Hz, C1), 150.01 (d, J_(PC)=4.60 Hz, C2), 152.52(2C, C11 and C13), 172.87 (C19); ³¹P NMR (121 MHz, {¹H}, D₂O) δ−2.61(s). Anal. Calcd for C₂₄H₃₀N₃O₁₀P.1.5 H₂O: C, 49.83; H 5.75; N,726. Found: C, 50.13; H, 5.78; N, 7.26.

The Karl Fisher and elemental analyses of the product of this procedureshowed that the crystalline salt is sesquihydrate. The DSC analysisindicated one major crystalline form with a melting endotherm at 158.6deg C. (see FIG. 5). The powder X-ray data obtained for this material isshown in FIG. 8, top pattern.

Differences of polymorphism were observed, at room temperature, as afunction of mmoles of CA4P free acid relative to the total volume of thecrystallization mixture. In the above procedure, 0.2 mmoles of CA4P freeacid per ml of the total volume of the crystallization mixture wereemployed. Modification of the above procedure such that 0.03 mmoles ofCA4P free acid per ml of the total volume of the crystallization mixturewere employed provided a CA4P mono-L-histidine salt form having 1.8molecules of water per molecule of salt (see FIG. 6, sample size 3.8500mg; the DSC analysis showed one crystalline form with a melthingendotherm at 184.9 deg C. ¹H NMR analysis showed the ratio ofCA4P:histidine=1:1). The powder X-ray data obtained for this material isshown in FIG. 8, bottom pattern. Another modification of the aboveprocedure such that 0.07 mmoles of CA4P free acid per ml of the totalvolume of the crystallization mixture were employed provided a mixtureof CA4P mono-L-histidine salt forms, one having 1.5 molecules of waterper molecule of salt, and the other having 1.8 molecules of water permolecule of salt (see FIG. 7, sample size 4.4500 mg; the Karl Fisher andelemental analyses of this mixture showed that the crystalline salt issesquihydrate. The DSC analysis illustrated two crystalline forms. Theendotherms are similar to those of FIGS. 5 and 6, respectively. Thepowder X-ray data obtained for this material is shown in FIG. 9. The DSCand powder X-ray data indicated that the 1.5:1 and 1.8:1 (water:salt)forms above are two different crystalline forms. As also noted above, amixture of these two forms is easily formed. With seeding, each form canbe made in a pure state.

The hemiheptahydrate CA4P mono-L-histidine salt form can also beobtained in the presence of water. This form, however, converts to thesesquihydrate CA4P mono-L-histidine salt form.

As explained above, a variety of natural or non-natural amino acidsincluding, but not limited to ornithine, lysine, arginine, andtryptophan can readily be substituted for histidine in the descriptiondescribed above to produce a compound of the present invention.

CA4P Mono-L-Histidine Hydrated Salt (Scale-Up)

L-histidine aqueous stock solution (0.2 M). L-Histidine (1.90 g, 12.0mmol) was dissolved in 60 mL of deionized water to form a 0.2 Msolution. (This solution can also be prepared in situ).

CA4P free acid stock solution in isopropyl alcohol (IPA) (0.17 M). CA4Pfree acid can be prepared in the following manner. The acid equivalentcan be reduced to 2.1; the addition of sodium chloride is not necessary.The following procedure is exemplary: CA4P disodium salt (8.94 g, 20.3mmol) was dissolved in 50 mL of deionized water. Ethyl acetate (200 mL)and a saturated aqueous sodium chloride solution (100 mL) were added tothe resulting solution with rapid stirring. A white cake was formed. Asolution of 0.5 N hydrochloric acid (220 mL) was added portion-wise todissolve the cake (the final pH of the aqueous phase was ca. 1 (pHpaper)). The organic phase was separated. The aqueous phase wasextracted with ethyl acetate (1×200 mL and then 2×150 mL). The organicphases were combined and dried over Na₂SO₄. Filtration and solventevaporation (rotavapor, water bath temperature=40° C.) yielded a thickfilm of CA4P free acid which was dissolved in 100 mL of IPA. Theconcentration of the resulting solution was determined to be 0.17 M by¹H NMR.

In order to confirm the concentration determined above, 60 μL ofhistidine solution (0.2 M) and 70 μL of CA4P free acid solution werepipetted into a 4-mL HPLC vial. The solvents were rotary evaporated todryness. The solid was dissolved in 0.7 mL of D₂O and analyzed by ¹H NMRto give a 1:1 ratio of histidine to CA4P free acid. A total of 17 mmolof CA4P free acid was obtained (84% yield).

CA4P mono-L-histidine hydrated salt (scale-up). A 250-mL round bottomflask was charged with 70.6 mL of CA4P free acid solution (0.17 M, 12.0mmol) and 50 mL of IPA. A solution of L-histidine (60 mL, 0.2 M, 12.0mmol) was added portion-wise to the CA4P free acid solution with rapidstirring. The resulting white slurry was stirred at 40° C. for 30 min,at ambient temperature for 3 h, followed by cooling to 0° C. (ice-bath)for 1 h. The crystalline solid was isolated by filtration through aWhatman #54 filter paper with suction, washed with cold isopropylalcohol, and dried in vacuo (vacuum desiccator) for 88 h to yield 6.07 gof CA4P mono-L-histidine as a white solid. Karl Fisher analysis of thesolid showed that the water content was 4.48%, which calculated to acrystalline solid hydrated with 1.5 water molecules per salt molecule(10.5 mmol, 87% yield): ¹H NMR (300 MHz, D₂O) δ 3.32 (d, J=6.6 Hz, 2H,C(21)H₂), 3.68 (s, 6H, C(15)H₃ and C(17)H₃), 3.74 (s, 3H, C(16)H₃), 3.82(s, 3H, C(18)H₃), 4.00 (t, J=6.6 Hz, 1H, C(20)H), 6.53 (d, J=12.1 Hz,1H, C(8)H), 6.62 (d, J=12.1 Hz, 1H, C(7)H), 6.64 (s, 2H, C(10)H andC(14)H), 6.95 (d, J=8.3 Hz, 1H, C(3)H), 7.02 (d, J=8.3 Hz, 1H, C(4)H),7.20 (broad s, 1H, C(6)H), 7.36 (broad s, 1H, C(23)H, 8.62 (d, J=1.3 Hz,1H, C(24)H); ¹³C NMR (100 MHz, {¹H}, D₂O) δ 26.11 (C21), 53.92 (C20),56.22 (2C, C15, C17), 56.32 (C18), 61.28 (C16), 106.82 (2C, C10, C14),113.20 (C3), 118.03 (C23), 122.01 (d, J_(PC)=2.3 Hz, C6), 125.48 (C4),127.78 (C22), 129.38 (C8), 129.97 (C7), 130.54 (C5), 133.92 (C9), 134.31(C24), 136.16 (C12), 141.38 (d, J_(PC)=6.1 Hz, C1), 149.98 (d,J_(PC)=5.4 Hz, C2), 152.48 (2C, C11 and C13), 172.86 (C19). Anal. Calcdfor C₂₄H₃₀N₃O₁₀P.1.5H₂O: C, 49.82; H, 5.75; N, 7.26; P, 5.35. Found: C,49.92; H, 5.84; N, 7.26; P, 5.44. Furthermore, using differentialscanning calorimetry, the compound obtained was determined to have amajor endotherm at 158° C., and a minor endotherm at 174° C.

Any suitable natural or non-natural amino acid can readily besubstituted into this procedure to produce other compounds of thepresent invention.

When CA4P mono-L-histidine salt is crystallized at room temperature,hydrate(s) are generally obtained. Carrying out the crystallizationprocess at temperatures elevated above room temperature, especiallygreater than 70° C., allows anhydrous salt to be obtained. Histidinesalt hydrates can be converted to the anhydrous crystalline form(especially, melting at 210° C.), for example, by slurrying a hydrateform in a solvent such as ethanol, methanol, isopropanol, or acetone, ata temperature such as 40° C. (such as for 2 days), followed byfiltration, washing and vacuum drying at a temperature such as 45° C.(such as overnight). The anhydrous form, which is practicallynonhygroscopic, is preferred.

CA4P Mono-L-Histidine Anhydrous Salt

A 200-mL round bottom flask was charged with L-histidine (0.2620 g, 1.65mmol) and 16.5 mL of deionized water. The resulting solution was heatedat 74-76° C. (oil bath temperature) with magnetic stirring. A solutionof CA4P free acid (8.7 mL, 0.19 M in IPA, 1.65 mmol) was added, followedby isopropyl alcohol (90 mL). The resulting solution became milky in ca.1 min. Stirring was continued at 75-76° C. for 2 h and then at ambienttemperature for 1 h. The needle crystalline solid was isolated byfiltration through a Whatman #4 filter paper with suction and dried in asteam of air (suction) overnight (19.5 h) and in vacuum desiccator for24 h to yield 0.7788 g of CA4P mono-L-histidine as a white solid (1.41mmol, 86% yield): mp 211.49° C. (DSC); ¹H NMR (400 MHz, D₂O) δ 3.30 (d,J=6.5 Hz, 2H, H-21), 3.65 (s, 6H, H-15 and H-17), 3.72 (s, 3H, H-16),3.80 (s, 3H, H-18), 3.99 (t, J=6.5 Hz, 1H, H-20), 6.50 (d, J=12.3 Hz,1H, H-8), 6.59 (d, J=12.3 Hz, 1H, H-7), 6.60 (s, 2H, H-10 and H-14),6.92 (d, J=8.5 Hz, 1H, H-3), 6.97 (broad d, J=8.5 Hz, 1H, H-4), 7.19(broad s, 1H, H-6), 7.33 (broad s, 1H, H-23), 8.58 (broad s, 1H, H-24);¹³C NMR (100 MHz, ({¹H}, D₂O) δ 27.11 (C-21), 54.88 (C-20), 57.17 (2C,C-15, and C-17), 57.24 (C-18), 62.24 (C-16), 107.77 (2C, C-10 and C-14),114.17 (C-3), 118.90 (C-23), 122.93 (d, J_(PC)=2.3 Hz, C-6), 126.40(C-4), 128.88 (C-22), 130.29 (C-8), 131.00 (C-7), 131.47 (C-5), 134.93(C-9), 135.32 (C-24), 137.08 (C-12), 14231 (d, J_(PC)=6.1 Hz, C-1),150.91 (d, J_(PC)=4.6 Hz, C-2), 153.45 (2C, C-11 and C-13), 173.84(C-19). Anal. Calcd for C₂₄H₃₀N₃O₁₀P: C, 52.27; H, 5.48; N, 7.62; P,5.61. Found: C, 52.03; H, 5.43; N, 7.57; P, 5.57.

CA4P Mono-L-Histidine Anhydrous Salt (scale-Up)

A 2000-mL three-neck round-bottom flask was equipped with a mechanicalstirrer, a 500-mL additional funnel, and a thermocouple which wasconnected to a Thermo-O-Watch L7-1100SA/28T which controlled a heatingmantle. L-histidine (3.42 g, 21.6 mmol) was added to the flask, followedby 216 mL of deionized water. The resulting solution was heated at74-80° C. with stirring. CA4P free acid (120 mL, 0.18 M in IPA, 21.6mmol) was added, followed by IPA (1176 mL), via the additional funnel ina rate to maintain the solution temperature at 73-74° C. (14 minrequired). After the addition of IPA, the resulting clear solution wasseeded with CA4P mono-L-histidine anhydrous salt (trace amount). Thesolution temperature was increased to 80° C., and crystallization tookplace in ca. 3 min after seeding. The temperature dropped down slowly to74° C. over 30 min and was maintained at 73-74° C. for another 1.5 h.The reaction mixture was allowed to cool down slowly to 31° C. in 3.5 h.The needle crystalline solid was filtered over a Whatman #4 filter paperwith suction, washed with IPA (100 mL), and dried in a stream of air(suction) overnight (16 h) and in a vacuum dessicator for 21.5 h toyield 10.11 g of CA4P mono-L-histidine anhydrous salt as white solid(18.3 mmol 85% yield): mp 213.65° C. (DSC); ¹H NMR (400 MHz, D₂O) δ 3.30(d, J=6.5 Hz, 2H, H-21), 3.65 (s, 6H, H-15 and H-17), 3.72 (s, 3H,H-16), 3.80 (s, 3H, H-18), 3.99 (t, J=6.5 Hz, 1H, H-20), 6.49 (d, J=12.0Hz, 1H, H-8), 6.58 (d, J=12.0 Hz, 1H, H-7), 6.59 (s, 2H, H-10 and H-14),6.91 (d, J=8.5 Hz, 1H, H-3), 6.97 (dd, J=8.3, 1.7 Hz, 1H, H-4), 7.19(broad t, J=1.7 Hz, 1H, H-6), 7.34 (broad s, 1H, H-23), 8.60 (d, J=1.4Hz, 1H, H-24); ¹³C NMR (100 MHz, {¹H}, D₂O) δ 27.07 (C-21), 54.86(C-20), 57.18 (2C, C-15 and C-17), 57.26 (C-18), 62.24 (C-16), 107.78(2C, C-10 and C-14), 114.18 (C-3), 118.94 (C-23), 122.95 (d, J_(PC)=2.3Hz, C6), 126.43 (C-4), 128.79 (C-22), 130.31 (C-8), 130.98 (C-7), 131.49(C-5), 134.91 (C-9), 135.29 (C-24), 137.10 (C-12), 142.31 (d, J_(PC)=6.9Hz, C-1), 150.92 (d, J_(PC)=4.6 Hz, C-2), 153.45 (2C, C-11 and C-13),173.82 (C-19). Anal. Calcd for C₂₄H₃₀N₃O₁₀P: C, 52.27; H, 5.48; N, 7.62;P, 5.61. Found: C, 52.23; H, 5.35; N, 7.60; P, 5.55.

The CA4P mono-L-histidine anhydrous salt can be made reproducibly as asingle crystalline form. FIG. 10 shows the DSC (sample size 2.3600 mg);FIG. 11 shows the powder X-ray data for this material.

Example 4 Preparation of cis-CA4P Monoglycine Methyl Ester Salt

The following procedure for the preparation of CA4P monoglycine methylester from CA4P disodium is advantageous. The procedure employs glycinemethyl ester hydrochloride directly in the presence ofN,N-diisopropylethylamine, providing enhanced stability as compared withpreparation employing glycine methyl ester free base. Furthermore, thepreparation of CA4P free acid is improved significantly—concentratedsulfinic acid is employed (rather than, for example, dilutedhydrochloride acid) in neutralization (as a result, the use of, forexample, ethyl acetate for extraction and then evaporation iseliminated). The formation of trans-CA4P free acid is avoided in thisimproved procedure.

Reagents and Method

The following reagents and chemicals were obtained from commercialsources and used without further purification: Isopropyl alcohol (IPA)(B&J Brand, High purity solvent grade), sulfuric acid (EM Science,95-98%, Lot #35310), glycine methyl ester hydrochloride (AldrichChemical Co. 99% labeled, Lot #03214 MU), N,N-diisopropylethylamine(Aldrich Chemical Co. 99.5% labeled, Lot #02819 ER). Multi-nuclei NMRspectra were recorded on Bruker DRX 400 spectrometer. The ¹H and ¹³C NMRchemical shifts are reported in ppm relative to tetramethylsilane (The¹³C NMR chemical shifts were determined using MeOH as externalstandard). The 2D NMR experiments [HMQC (Heteronuclear Multiple QuantumCorrelation spectroscopy, an inverse chemical shift correlationexperiment to determine which ¹H's of the molecule are bonded to which¹³C nuclei (or other X nuclei)]; and HMBC (Heteronuclear Multiple BondCorrelation spectroscopy, a modified version of HMQC suitable todetermine long-range ¹H-¹³C connectivity, as well as the structure and¹H and ¹³C assignments of the molecule)) were conducted to aid theassignments of the ¹H and ¹³C NMR signals to the structure. Differentialscanning calorimetry (DSC) was performed on DSC 2920 DifferentialScanning Calorimeter, TA Instruments.

Cis-CA4P Monoglycine Methyl Ester Salt

A 100-mL round-bottom flask was charged with cis-CA4P disodium salt(2.866 g, 6.51 mmol) and EPA (30 mL). The resulting slurry wasmagnetically stirred at ambient temperature. A solution of sulfuric acid(0.365 mL, 6.51 mmol) in IPA (60 mL) was added portionwise to theslurry. The mixture was continuously stirred for about 10 min andfiltered with suction using a Whatman #1 filter paper. The solid (Na₂SO₄which is insoluble in IPA) was washed with IPA (10 mL). The filtrate andwash, which contained CA4P free acid, were combined in another 100-mLround-bottom flask. Glycine methyl ester hydrochloride (0.826 g, 6.51mmol) and N,N-diisopropylethylamine (1.254 mL, 7.16 mmol) were added tothe combined solution. The resulting mixture was heated in an oil bathwith magnetically stirring. At 60° C., the mixture became a clearsolution. At 65° C., the solution became slurry. At 78° C., the slurrydissolved to form a clear solution. Heating was stopped and the solutionwas allowed to cool down slowly in the oil bath. At 60° C., the seed ofcis-CA-4P monoglycine methyl ester salt was added to the solution toform a slurry. Stirring was continued from 60° C. to ambient temperaturefor about 1 h and then at ambient temperature overnight. The whitecrystalline solid was isolated by filtration with suction using aWhatman #1 filter paper and washed with IPA (3×10 mL) and dried in astream of air for 6 h to give 2.609 g of cis-CA4P monoglycine methylester salt (5.38 mmol, 82.6% yield): HPLC analysis, 100% cis-CA4P; mp136.40° C. (DSC); ¹H NMR (400 MHz, D₂O) δ 3.52 (s, 6H, H-15 and H-17),3.61 (s, 3H, H-16), 3.71 (s, 3H, H-18), 3.77 (s, 3H, H-21), 3.87 (s, 2H,H-20), 6.26 (d, J=12.1 Hz, 1H, H-8), 6.42 (s, 2H, H-10 and H-14), 6.43(d, J=12.1 Hz, 1H, H-7), 6.70 (d, J=8.8 Hz, 1H, H-3), 6.79 (broad d,J=8.8 Hz, 1H, H-4), 7.16 (broad s, 1H, H-6); ¹³C NMR (100 MHz {¹H}, D₂O)δ 41.27 (C-20), 54.58 (C-21), 56.99 (2C, C-15, and C-17), 57.21 (C-18),62.09 (C-16), 107.60 (2C, C-10 and C-14), 113.89 (C-3), 122.98 (C-6),126.22 (C4), 130.25 (C-8), 130.69 (C-7), 131.37 (C-5), 134.61 (C-9),137.09 (C-12), 142.42 (d, ²J_(PC)=6.9 Hz, C-1), 150.89 (d, ³J_(PC)=4.6Hz, C-2), 153.33 (2C, C-11 and C-13), 169.95 (C-19). Anal. Calcd forC₂₁H₂₈NO₁₀P: C, 51.96; H, 5.81; N, 2.88; P, 6.38. Found: C, 51.74; H,5.79; N, 2.87; P, 6.30.

(Note: Numbering systems as shown above, and wherever such numberingsystems are shown herein, are for convenience only and may not beconsistent with IUPAC nomenclature).

The DSC of the cis-CA4P monoglycine methyl ester salt (sample size:3.7400 mg) is shown in FIG. 12; FIG. 13 shows the powder X-ray data forthis material (2 batches).

Example 5 Preparation of the Glycine Ethyl Ester Salt of CA4P Free Acid

Ethyl acetate (2 mL), CA4P isopropanol solution (150 micro L of a 0.42 Msolution, 63 micro mol) and glycine ethyl ester methyl tert-butyl ethersolution (800 micro L of a 0.08 M solution, 64 micro mol) were added toan HPLC vial and agitated vigorously for ˜3 minutes. The resulting clearsolution was seeded with a drop of slurry from another experiment andthe mixture was allowed to stand overnight at ambient temperature. Awhite solid formed that did not appear to be crystalline based uponmicroscopic examination and so the mixture was allowed to stand atambient temperature for three more days. Methyl tert-butyl ether (1 mL)was added and the mixture was stirred for about 10 minutes. Microscopicexamination of the resulting mixture indicated that the solid hadconverted to a crystalline needles. The needles were isolated by vacuumfiltration and dried to afford the glycine ethyl ester salt of CA4P(22.4 mg, 66 M % yield). Proton NMR analysis indicated that the ratio ofglycine ethyl ester to CA4P was 1.7:1. ¹H NMR data for CA4P glycineethyl ester.

¹H NMR (300 MHz, D₂O) δ1.20 (t, J=7.2 Hz, 3H, CH₃), 3.60 (s, 6H, H-15and H-17), 3.66 (s, 3H, H-16), 3.74 (s, 3H, H-18), 3.79 (s, 2H, CH₂N),4.21 (q, J=7.2 Hz, 2H, CH₂CH₃), 6.44 (d, J=12.2 Hz, 1H, H-8), 6.55 (d,J=12.2 Hz, 1H, H-7), 6.57 (s, 2H, H-10 and H-14), 6.80 (d, J=8.7 Hz, 1H,H-3), 6.85 (broad dl, J=8.7 Hz, 1H, H-4), 7.23 (broad s, 1H, H-6).

The present invention is not to be limited in scope by the specificembodiments described herein. Indeed, various modifications of theinvention in addition to those described herein will become apparent tothose skilled in the art from the foregoing description. Suchmodifications are intended to fall within the scope of the appendedclaims.

Various publications and patent documents are cited herein, thedisclosures of which are incorporated by reference in their entireties.

1. A crystalline form of combretastatin A4 phosphate mono-tromethamine.2. The crystalline form of claim 1, wherein the olefin of the compoundis in the cis configuration.
 3. The crystalline form of claim 1 that hasless than 1% water weight gain per weight of compound.
 4. Thecrystalline form of claim 1, wherein the form is an achiral neat form(N−I), which does not contain any solvent sites.
 5. The crystalline formof claim 1, wherein the differential scanning calorimetry thermogramshows a single melt exotherm at 196° C.
 6. The crystalline form of claim1, wherein thermogravimetric analysis does not show weight losses below150° C.
 7. The crystalline form of claim 1, wherein equilibrium aqueoussolubility at 25° C. is 3.37 mg/mL at pH 4.8.
 8. The crystalline form ofclaim 1 having a pH-solubility profile as shown in FIG.
 4. 9. Thecrystalline form of claim 1 having a moisture sorption/desorptionprofile as shown in FIG. 1.